Dataset Information

A multidisciplinary integrated approach for the identification and characterization of the AMP profile in Hermetia illucens hemolymph

ABSTRACT: The growing threat of antimicrobial resistance calls for alternative strategies to conventional antibiotics. Insects represent a promising source of antimicrobial peptides (AMPs) due to their potent innate immune responses. In this study, we investigated the peptide extracts from hemolymph of Hermetia illucens larvae as a bioresource of infection-induced AMPs. Larvae were challenged with Gram-negative (Escherichia coli) and Gram-positive (Micrococcus flavus) bacteria, and hemolymph-derived peptides were extracted and fractionated via reverse-phase high-performance liquid chromatography (RP-HPLC). Antibacterial activity of peptide fractions was evaluated in vitro against a panel of pathogenic strains, including multidrug-resistance isolated such as carbapenem-resistant Klebsiella pneumoniae. The peptide fractions were further analyzed by SDS-PAGE and LC-MS/MS to identify candidate AMPs. In parallel, shotgun proteomics on unfractionated peptide extracts enabled quantitative comparison across experimental conditions. In silico analysis using ProP 1.0, APD3, and CAMPR4 prediction tools revealed physicochemical profiles and machine learning-based scores consistent with antimicrobial function. Our integrative approach confirms that bacterial infection induces a diverse and bioactive immunopeptidome in H. illucens, supporting its potential as a sustainable platform for discovery of novel antimicrobial agents.

INSTRUMENT(S):

ORGANISM(S): Hermetia Illucens

TISSUE(S): Hemolymph

SUBMITTER: ilaria iacobucci

LAB HEAD: Maria Monti

PROVIDER: PXD071144 | Pride | 2026-06-01

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	EXPHERMETIA_COLI_1_REP1_15MAG24.raw	Raw
	EXPHERMETIA_COLI_1_REP2_15MAG24.raw	Raw
	EXPHERMETIA_COLI_2_REP1_15MAG24.raw	Raw
	EXPHERMETIA_COLI_2_REP2_15MAG24.raw	Raw
	EXPHERMETIA_COLI_3_REP1_15MAG24.raw	Raw

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Publications

A Multidisciplinary Integrated Approach for the Identification and Characterization of the AMP Profile in Hermetia illucens Hemolymph.

De Stefano Federica F Monaco Vittoria V Giglio Fabiana F Scieuzo Carmen C Rinaldi Roberta R Salvia Rosanna R Quaranta Gianluca G Amaro Sofia S Fusco Alessandra A Mancini Ignazio Marcello IM Monti Maria M Donnarumma Giovanna G Falabella Patrizia P

Insects 20260509 5

The growing threat of antimicrobial resistance necessitates alternative strategies to conventional antibiotics. Insects represent a promising source of antimicrobial peptides (AMPs) due to their potent innate immune responses. In this study, we investigated the hemolymph peptide extracts from Hermetia illucens larvae as a bioresource of infection-induced AMPs. Larvae were challenged with Gram-negative (Escherichia coli) and Gram-positive (Micrococcus flavus) bacteria, and he ...[more]

PMID: 42188153

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Project description:Antimicrobial peptides (AMPs) represent promising alternatives to conventional antibiotics in the fight against multidrug-resistant pathogens. Here, we characterize the antibacterial properties and molecular mode of action of the C-terminal fragment of the Medicago truncatula nodule-specific cysteine-rich peptide NCR169 (NCR169C17-38). This peptide exhibits strong bactericidal activity against a broad panel of Gram-positive and Gram-negative pathogens, including members of the ESKAPE group (Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli). NCR169C17-38 acted rapidly by permeabilizing bacterial membranes and causing severe morphological damage, outperforming polymyxin B in the rate of membrane disruption. The peptide bound selectively to anionic bacterial lipids such as cardiolipin and displayed remarkable thermal and storage stability. Moreover, NCR169C17-38 effectively inhibited biofilm formation and eradicated pre-formed biofilms of Acinetobacter baumannii even at sub-MBC concentration (3.2 M), a feature not observed for polymyxin B. Transcriptomic analysis of Escherichia coli exposed to sublethal peptide doses revealed a global shutdown of bacterial metabolic and genetic functions, with 450 of 503 differentially expressed genes being downregulated. Strong repression of genes involved in translation, energy production, and cell envelope biosynthesis was accompanied by upregulation of stress- and rescue-related genes linked to membrane repair, ion transport, and oxidative stress responses. Together, these results demonstrate that NCR169C17-38 exerts multifaceted antimicrobial effects - disrupting membranes, binding nucleic acids, and inducing transcriptional collapse - while maintaining its stability and biocompatibility. Together with its previously established antifungal activity and lack of mammalian cytotoxicity, NCR169C17-38 represents a compelling candidate for development as a next-generation antimicrobial agent.

Dataset Information

A multidisciplinary integrated approach for the identification and characterization of the AMP profile in Hermetia illucens hemolymph

Dataset's files

Publications

A Multidisciplinary Integrated Approach for the Identification and Characterization of the AMP Profile in <i>Hermetia illucens</i> Hemolymph.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

A multidisciplinary integrated approach for the identification and characterization of the AMP profile in Hermetia illucens hemolymph

Dataset's files

Publications

A Multidisciplinary Integrated Approach for the Identification and Characterization of the AMP Profile in &lt;i&gt;Hermetia illucens&lt;/i&gt; Hemolymph.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

A Multidisciplinary Integrated Approach for the Identification and Characterization of the AMP Profile in <i>Hermetia illucens</i> Hemolymph.