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Quantitative DIA/SWATH proteomic profiling of SAGA complex mutants in Cryptococcus neoformans

ABSTRACT: This project investigates the proteomic consequences of deleting key subunits of the SAGA transcriptional coactivator complex (Hfi1, Spt7 and Spt20) in Cryptococcus neoformans. Quantitative SWATH/DIA LC-MS/MS was performed on wild-type and mutant strains grown at 30°C and 37°C to identify pathway-level alterations associated with impaired SAGA function. The dataset provides peptide- and protein-level quantification to support differential abundance analysis and functional interpretation of SAGA-dependent regulatory processes.

INSTRUMENT(S):

ORGANISM(S): Fungi Cryptococcus Neoformans Var. Grubii Serotype A (strain H99 / Atcc 208821 / Cbs 10515 / Fgsc 9487) (filobasidiella Neoformans Var. Grubii)

TISSUE(S): Cell Culture

SUBMITTER: Chendi Yu

LAB HEAD: James Fraser

PROVIDER: PXD071194 | Pride | 2026-01-03

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20240326_SWATH_output_Lib.tsv	Tabular
	WT_30C_rep1.wiff	Wiff
	WT_30C_rep1.wiff.scan	Wiff
	WT_30C_rep1.wiff2	Wiff
	WT_30C_rep2.wiff	Wiff

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Coactivator SAGA plays a crucial role in TBP recruitment for the Gcn4 transcriptome via subunits Spt3/Spt8 [RNA-seq]

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