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Proteomic analysis of Src cells treated with the Src inhibitor PP2

ABSTRACT: This project is focus on the role of Src signaling involved in translation related to invadosome formation. The aim of this project is to decipher the molecular mechanism involved in specific and local translation into invadosome.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Dupuy Jean-William

LAB HEAD: Frederic Saltel

PROVIDER: PXD071367 | Pride | 2025-11-28

REPOSITORIES: Pride

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			Action	DRS
	F013694.dat	Other
	F013695.dat	Other
	F013696.dat	Other
	F013697.dat	Other
	F013698.dat	Other

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Combining laser capture micro-dissection and proteomics reveals an active translation machinery controlling invadosome formation

Project description:We developed a method that combines laser micro-dissection and mass spectrometry, enabling the analysis of subcellular structures in their native state based on low amounts of input material. Using this combinatorial method, we showed that invadosomes contain specific components of the translational machinery, in addition to known marker proteins.

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Proteomic analysis of bronchial smooth muscle cells extracellular vesicles

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Analyses of FBXL6 interaction with newly synthesized proteins.

Project description:HEK cells were grown in light media for two weeks. One day before experiments, cells were transfected with FBXL6-flag or B-gal (control). At t=0, cells were switched to heavy media for 3h and then Flag immunoprecipitation were performed. Pulled down protein were analyzed by mass spectrometry.

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Proteomic analysis of WT and RagA-deficient macrophages that have phagocytosed SILAC-bacteria

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Proteomic analysis of macrophages that have phagocytosed SILAC -bacteria using a trans-well system

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Proteomic analysis of lungs in early experimental model of Chronic obstructive pulmonary disease (COPD)

Project description:Rationale: Chronic obstructive pulmonary disease (COPD) is a prevalent respiratory disease lacking disease modifying treatment. The role of CXCL12/CXCR4 axis has been demonstrated in acute exacerbation of COPD. The interest of study early COPD has been recently pointed out. Objectives: To study the role of the CXCL12/CXCR4 axis in both human and mouse model of early COPD. Methods: Blood and lung tissue were obtained from both COPD patients and mice exposed to cigarette smoke (CS) for 10 weeks and intranasal instillations of polyinosinic–polycytidylic acid (poly(I:C)) to mimic exacerbations for 5 weeks. Measurements and Main Results: Exposed mice presented mild airway obstruction, peri-bronchial fibrosis and right heart remodeling. The level of CXCR4 expressing cells was increased in the blood of exposed mice, as well as in the blood of patients with mild COPD. Lung CXCL12 expression was higher both in exposed mice and COPD patients. The densities of fibrocytes expressing CXCR4 were increased in the blood and in the bronchial submucosa of exposed mice. Conditional inactivation of CXCR4 at adult stage as well as pharmacological inhibition of CXCR4 with plerixafor injections improved lung function and inflammation and protected against CS and poly-(I:C)-induced airway and cardiac remodeling. CXCR4-/- and plerixafor-treated mice also had reduced levels of CXCR4-expressing circulating cells and a lower density of peri-bronchial fibrocytes. Conclusions: We demonstrated that targeting CXCR4 has beneficial effects in an animal model of early COPD, and provide a framework to translate preclinical findings to clinical settings within a drug repurposing approach.

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Proteomic analysis of HepG2 cells after mutated ß-catenin depletion

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