Proteomics

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Proteomic and Metabolomic Profiling of Invasive Methicillin Resistant Staphy-lococcus aureus : Uncovering Key Biomarkers and Pathogenic Pathways

ABSTRACT: Understanding the behavioural differences between methicillin-resistant Staphylococcus aureus (MRSA) associated with invasive versus non-invasive infections is key to unraveling their infection mechanisms and identifying biomarkers with potential translational value. This study aimed to compare the proteomic and metabolomic profiles of MRSA isolates derived from different infections to uncover distinct molecular signatures. MRSA isolates from invasive infections (blood cultures, n = 23), non-invasive infections (superficial skin, n = 49), and colonization (nasal swabs, n = 24) were analyzed Using the dual functionality methanol extraction method, proteins and metabolites were extracted from bacterial colonies grown on blood agar. Proteins were analyzed using the Orbitrap Exploris 480, and metabolites were characterized using a TimsTOF mass spectrometer with an Apollo II electrospray ionization source. Data was acquired using DIA, and the peptides were assigned to their respective proteins using DIA-NN, while metabolomic data analysis was conducted using MetaboScape® 4.0. A total of 2000 proteins and 150 metabolites were identified across all the MRSA isolates. No significant differences in protein or metabolite changes were observed between nasal colonizers and isolates from non-invasive infections. However, compared with isolates from non-invasive infections and colonization, isolates from invasive infections consistently exhibited higher levels of two metabolites (Sphinganine and Phosphoserine) and one protein (Staphylococcal secretory antigen ssaA2). Conversely, three metabolites (Cytidine, Benzoic acid, and Guanosine) and two proteins (Small ribosomal subunit protein bS20 and Bifunctional autolysin) were found at significantly lower intensities in isolates from invasive infections.. Over-representation analysis of enriched KEGG pathways revealed significant enrichment of the sphingolipid metabolism and ribosomal pathways (p<0.05).

INSTRUMENT(S):

ORGANISM(S): Staphylococcus Aureus

TISSUE(S): Cell Culture

SUBMITTER: Alexander Giddey  

LAB HEAD: Abiola Senok

PROVIDER: PXD071424 | Pride | 2026-06-08

REPOSITORIES: Pride

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CGM_AS_007_20231124_10uL_BUR-11.mzML Mzml
CGM_AS_007_20231124_10uL_BUR-20.mzML Mzml
CGM_AS_007_20231124_10uL_BUR-21.mzML Mzml
CGM_AS_007_20231124_10uL_BUR-24.mzML Mzml
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Proteomic and metabolomic profiling of methicillin-resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; associated with invasive vs. non-invasive infections: uncovering key biomarkers and pathogenic pathways.

Boucherabine Syrine S   Giddey Alexander D AD   Nassar Rania R   Mohamed Lobna L   Verma Subham S   Soares Nelson C NC   Senok Abiola A  

Frontiers in microbiology 20260506

<h4>Introduction</h4>Understanding the behavioral differences between invasive and non-invasive methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is essential for unraveling infection mechanisms and identifying biomarkers with translational potential. This study compared the proteomic and metabolomic profiles of MRSA isolates from diverse clinical presentations to uncover distinct molecular signatures.<h4>Methods</h4>Invasive isolates were obtained from blood cultures (<i>n</i> = 23), wh  ...[more]

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