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Secretome proteomics of THP-1 macrophages under M0, M1, and M2 polarization

ABSTRACT: This dataset comprises label-free quantitative proteomics of the secretome from human THP-1 serglycin (SRGN) knock-out and wild-type macrophage-like cells differentiated with PMA (M0) and polarized to M1 (LPS + IFN-γ) or M2 (IL-4 + IL-13). The study investigates how macrophage activation states in the absence of serglycin reshape the composition of secreted proteins relevant to inflammatory signaling, vesicle biology, and metabolism. Conditioned media were collected after polarization, processed by bottom-up proteomics, and analyzed by nanoLC–MS/MS on an Orbitrap Fusion Lumos. The resulting protein abundance profiles provide a resource to compare resting (M0) versus pro-inflammatory (M1) and alternatively activated (M2) macrophage secretomes in a standardized cell system.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte

SUBMITTER: Erik Knutsen

LAB HEAD: Erik Knutsen

PROVIDER: PXD071481 | Pride | 2026-02-25

REPOSITORIES: Pride

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			Action	DRS
	231208_EKnutsen_THP1_KO_M0_S16.raw	Raw
	231208_EKnutsen_THP1_KO_M0_S22.raw	Raw
	231208_EKnutsen_THP1_KO_M0_S4.raw	Raw
	231208_EKnutsen_THP1_KO_M1_S17.raw	Raw
	231208_EKnutsen_THP1_KO_M1_S23.raw	Raw

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