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Multi‑instrument DIA proteomics of Escherichia coli and Halomonas bluephagenesis TD01 ratio mixtures for biomanufacturing‑relevant instrument comparison

ABSTRACT: This project benchmarks five DIA-based LC–MS platforms for their ability to quantify proteins in E. coli and Halomonas bluephagenesis under biomanufacturing‑relevant conditions. Using simple peptide ratio mixtures and an exemplar carbon source comparison, it shows how instruments across a range of costs can still deliver actionable coverage of core metabolic pathways. The work is aimed at positioning quantitative proteomics as a practical tool for chassis selection, process development, and troubleshooting in industrial biomanufacturing.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli Halomonas Bluephagenesis

SUBMITTER: Matthew Russell

LAB HEAD: Nigel Scrutton

PROVIDER: PXD071869 | Pride | 2026-01-09

REPOSITORIES: Pride

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			Action	DRS
	01_SWATH_2E_1H.timeseries.data	Other
	01_SWATH_2E_1H.wiff	Wiff
	01_SWATH_2E_1H.wiff.scan	Wiff
	01_SWATH_2E_1H.wiff2	Wiff
	02_SWATH_1E_1H.timeseries.data	Other

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