Dataset Information

Proteomic profiling of frozen and lyophilized bovine milk-derived extracellular vesicles using LC-MS/MS

ABSTRACT: This project aimed to compare the proteomic landscapes of bovine milk-derived extracellular vesicles (EVs) preserved under two storage conditions—lyophilization and conventional freezing at −80 °C. By evaluating how different preservation strategies influence EV protein composition, the study sought to determine whether lyophilization can serve as a practical and reliable alternative to low-temperature storage for maintaining EV integrity and functionality.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Milk

SUBMITTER: Seyed Omid Reza Mousavi

LAB HEAD: Alireza Fazeli

PROVIDER: PXD071883 | Pride | 2026-01-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	240905_04_Orbi3_MS_SER_G60_T85_Fazeli_Subhashini_FW_3.raw	Raw
	240905_06_Orbi3_MS_SER_G60_T85_Fazeli_Subhashini_FW_1.raw	Raw
	240905_08_Orbi3_MS_SER_G60_T85_Fazeli_Subhashini_FW_4.raw	Raw
	240905_10_Orbi3_MS_SER_G60_T85_Fazeli_Subhashini_FW_2.raw	Raw
	240905_12_Orbi3_MS_SER_G60_T85_Fazeli_Subhashini_LW_4.raw	Raw

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Publications

Milk-Derived Extracellular Vesicles Protect Bovine Oviduct Epithelial Cells from Oxidative Stress.

Mousavi Seyed Omid Reza SOR Reshi Qurat Ul Ain QUA Godakumara Kasun K Muhandiram Subhashini S Midekessa Getnet G Andronowska Aneta A Kopanchuk Sergei S Lavogina Darja D Rinken Ago A Kodithuwakku Suranga S Fazeli Alireza A

Cells 20251222 1

Extracellular vesicles (EVs) are promising therapeutic agents due to their role in intercellular communication. This study examined the protective effects of milk-derived EVs (mEVs) on bovine oviductal epithelial cells (BOECs) under cobalt chloride (CoCl2)-induced oxidative stress (OS), comparing EVs stored at -80 °C or lyophilized. mEVs and algae-derived EVs (aEVs; negative control) were isolated via tangential flow filtration and applied at 107, 109, and 10<sup ...[more]

PMID: 41511305

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