Dataset Information

Type B gelatin obtained by ultrasound-assisted extraction from Nile tilapia (Oreochromis niloticus) scales.

ABSTRACT: mechanical evaluation of a biomaterial composed of gelatin and hydroxyapatite obtained from fish waste.

INSTRUMENT(S):

ORGANISM(S): Oreochromis Niloticus

SUBMITTER: Pablo Juanes-Velasco

LAB HEAD: Mario Enrique Velásquez Lozano

PROVIDER: PXD071911 | Pride | 2026-05-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1_HGE_1_S1-A8_1_2037.d.zip	Other
	1_HGE_1_S1-A8_1_2037.txt	Txt
	1_HGE_2_S1-A9_1_2038.d.zip	Other
	1_HGE_2_S1-A9_1_2038.txt	Txt
	1_HGE_3_S1-A10_1_2039.d.zip	Other

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Publications

Physicochemical and Functional Properties of Type B Gelatin Obtained from Nile Tilapia (Oreochromis niloticus) Scales Using Hydro-Extraction: Effect of Ultrasound Pretreatment.

Triana-Jiménez Kelly K Alonso Carlos C Vega Milena A MA Juanes-Velasco Pablo P Menéses-Rivera Iván I Velásquez-Lozano Mario M

Pharmaceutics 20260409 4

Background: In this study, type B gelatin was extracted from Oreochromis niloticus scales under hydrothermal conditions at 60 °C to evaluate the effect of ultrasound-assisted pretreatment on its structural, physicochemical, thermal, and functional properties. Methods: Gelatin obtained with and without ultrasound pretreatment was systematically characterized through molecular weight analysis, proteomic profiling, size determination, surface morphology, proximate composition, ...[more]

PMID: 42076115

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Project description:Predicting male fertility remains a major challenge in livestock industry. Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that are involved in the regulation of sperm functionality and may therefore be involved in male fertility. The aim of this study was to evaluate whether the protein load of sEVs differs between boars showing differences in fertility when used for artificial insemination (AI). An in-depth quantitative proteomic analysis was performed on two subsets of sEVs of different size (small [S] and large [L]) isolated from boars with different reproductive performance as assessed by farrowing rate (FR) and litter size (LS). Seminal plasma samples from 18 AI-boars (three per boar) from four fertility groups: high FR (H-FR, n=5), low FR (L-FR, n=5), large LS (L-LS, n=4), and small LS (S-LS, n=4) were used. Seminal plasma samples from each fertility group were randomly mixed resulting in three seminal plasma pools per fertility group. Seminal EVs were isolated by size exclusion chromatography and characterized according to MISEV2023 guidelines. Proteomic analysis was performed using a Bruker timsTOF fleX™ instrument with dia PASEF technology. A total of 470 and 726 proteins were quantified in S-sEVs, and 1,801 and 1,834 proteins in L-sEVs from boars with differences in FR and LS, respectively. Differential protein abundance analysis revealed three and 12 proteins quantified (P ≤ 0.05) only in S-sEVs and L-sEVs from L-FR boar samples, and 37 and five proteins quantified (P ≤ 0.05) only in S-sEVs and L-sEVs from L-LS boar samples, respectively. For proteins quantified in all fertility groups, the comparison between L-FR and H-FR boar samples revealed 4 and 40 differentially abundant proteins (DAPs; log2 fold change ≥ ±1, P ≤ 0.05) in S-sEVs and L-sEVs, respectively. When comparing L-LS and S-LS boar samples, 10 and 47 DAPs were identified in S-sEVs and L-sEVs, respectively. These results show that the protein load of sEVs varies between more and less fertile boars and that the DAPs are different between S-sEVs and L-sEVs. These results would support that sEVs proteins would be involved in the fertility of boars and that those that are exclusively and/or differentially abundant between more and less fertile boars could be candidates for fertility biomarkers

Dataset Information

Type B gelatin obtained by ultrasound-assisted extraction from Nile tilapia (Oreochromis niloticus) scales.

Dataset's files

Publications

Physicochemical and Functional Properties of Type B Gelatin Obtained from Nile Tilapia (<i>Oreochromis niloticus</i>) Scales Using Hydro-Extraction: Effect of Ultrasound Pretreatment.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Type B gelatin obtained by ultrasound-assisted extraction from Nile tilapia (Oreochromis niloticus) scales.

Dataset's files

Publications

Physicochemical and Functional Properties of Type B Gelatin Obtained from Nile Tilapia (&lt;i&gt;Oreochromis niloticus&lt;/i&gt;) Scales Using Hydro-Extraction: Effect of Ultrasound Pretreatment.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Physicochemical and Functional Properties of Type B Gelatin Obtained from Nile Tilapia (<i>Oreochromis niloticus</i>) Scales Using Hydro-Extraction: Effect of Ultrasound Pretreatment.