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Food Dependent Activation of One Carbon Metabolism During Heat Shock in Mytilus californianus

ABSTRACT: Of the ecological factors affecting the cellular stress response (CSR), food ration has received little attention despite a likely role in stress tolerance limits. To test the interactive effects of food and temperature on the proteomic response to heat shock, the intertidal mussel Mytilus californianus was acclimated to four combinations of nearshore (low) and aquaculture (high) phytoplankton levels, combined with low (20 °C) and high (30 °C) aerial temperatures during daytime low tides. Whole mussels were then exposed to an acute (6 h), aerial heat stress (33 °C) and allowed to recover for 1 h and 24 h in pre-exposure conditions. Proteomic changes in the gill before and after heat shock were measured using liquid-chromatography-mass spectrometry and label-free quantification. Mussels acclimated to low-temperature-low-food (LTLF) elicited a greater heat shock response compared to other acclimation groups, increasing protein levels of core carbohydrate and sulfide metabolism. These glycolytic metabolites proposedly feed into one-carbon metabolism (e.g., folate and methionine cycles) and connecting pathways, which also increased abundance and likely contribute to purine synthesis, methylation, and transsulfuration. These mussels additionally increased levels of proteins scavenging hydrogen peroxide, chelating ferrous iron, and maintaining reduced glutathione levels. Proteins modifying chromatin (histone) structure and regulating transcription likely link the metabolic changes to chromatin modifications, specifically through SAM-dependent methyltransferases, which may contribute to repairing DNA-damage caused by oxidative stress. Overall, our results suggest a food-dependent link between metabolism and epigenetic modifications that we hypothesize may function as an autoregulatory feedback mechanism during the CSR under tidally fluctuating conditions. SUMMARY STATEMENT A food-dependent link between metabolism and epigenetic modifications may function as an autoregulatory feedback mechanism to repair DNA damage and scavenge oxidants during heat stress.

INSTRUMENT(S):

ORGANISM(S): Mytilus Californianus

TISSUE(S): Gill

SUBMITTER: Frank Fabela

LAB HEAD: Lars Tomanek

PROVIDER: PXD072074 | Pride | 2026-07-28

REPOSITORIES: Pride

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Source:

			Action	DRS
	20231007_CWF_FF.pdConsensusWF	Other
	ApQuantWorkflow_No_Spectral_Clustering_FF20231009.pdProcessingWF	Other
	BL2_5_20_H1_1.raw	Raw
	BL2_5_20_H1_2.raw	Raw
	BL2_5_20_H1_3.raw	Raw

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Food Dependent Activation of One Carbon Metabolism During Heat Shock in Mytilus californianus

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