Dataset Information

Protein interactors of 5-FU in MDA-MB-231 cells

ABSTRACT: This dataset originates from a Proteome Integral Solubility Alteration (PISA) experiment designed to assess how 5-fluorouracil (5-FU) alters protein thermal stability/solubility in MDA-MB-231 cells. Cells were treated with 200 μM 5-FU or DMSO for 2 h, exposed to a temperature gradient, lysed, and soluble proteins from each condition were isolated, digested, and labeled with TMTpro reagents. Labeled peptides were fractionated and analyzed by LC-MS/MS. The raw data were processed with MaxQuant against the human UniProt reference proteome, providing proteome-wide quantitative profiles of 5-FU-induced changes in protein solubility.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mda-mb-231 Cell

SUBMITTER: Remigiusz Serwa

LAB HEAD: Dr. Abdelhalim Azzi

PROVIDER: PXD072095 | Pride | 2026-02-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	HA_PISA_TMT13_fr1.raw	Raw
	HA_PISA_TMT13_fr1_20240309004042.raw	Raw
	HA_PISA_TMT13_fr2.raw	Raw
	HA_PISA_TMT13_fr2_20240309070412.raw	Raw
	HA_PISA_TMT13_fr3.raw	Raw

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Publications

The mTORC2 component SIN1 post-transcriptionally regulates TYMS levels and modulates P53 activity in response to 5-FU chemotherapy.

Sayed Abdulrahman El AE Gomes Nelson N Zakrzewski Maciej M Liudkovska Vladyslava V Serwa Remigiusz R Azzi Abdelhalim A

Cell communication and signaling : CCS 20260128 1

PMID: 41606600

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