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Copper-responsive proteome changes in human kidney proximal tubule epithelial cells

ABSTRACT: Copper is essential for cellular function but potentially toxic in excess. While copper's influence on cytoskeletal dynamics remains unclear, loss of mechanical integrity is a hallmark of kidney disease. Here we investigated how copper availability modulates mechanical properties and cytoskeletal architecture of human proximal tubule epithelial cells (HK-2). Cells were treated for 24h with 50 µM CuCl₂ (copper supplementation) or 10 µM tetrathiomolybdate (TTM, copper chelation). Quantitative proteomics revealed coordinated changes in actin-regulatory proteins including Rab35, Septin2, MsrB2, and CK2 subunits, establishing redox-dependent pathways linking copper homeostasis to cytoskeletal dynamics. A total of 4,933 proteins were quantified with 89-90% data completeness, including detection of 13,919 PTMs per run (carbamidomethylation, oxidation, phosphorylation sites)

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell Of Proximal Tubule, Kidney

SUBMITTER: Rafael Valverde

LAB HEAD: Marcelo Einicker Lamas

PROVIDER: PXD072220 | Pride | 2026-07-21

REPOSITORIES: Pride

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			Action	DRS
	HK-2_Control_1.raw	Raw
	HK-2_Control_3.raw	Raw
	HK-2_Control_4.raw	Raw
	HK-2_Copper_1.raw	Raw
	HK-2_Copper_2.raw	Raw

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