Dataset Information

Shotgun proteomic analysis of the marine bacteria Alcanivorax sp. 24 and Alcanivorax borkumensis SK2 grown with weathered polyethylene.

ABSTRACT: Cell proteomes obtained from Alcanivorax sp. 24 and Alcanivorax borkumensis SK2 grown with pyruvate, weathered polyethylene (W-PE) and hexadecane were analysed to investigate de bacterial metabolism for W-PE assimilation.

INSTRUMENT(S):

ORGANISM(S): Bacteria Alcanivorax Sp. 24 Alcanivorax Borkumensis Sk2

SUBMITTER: Theo Obrador-Viel

LAB HEAD: Joseph Alexander Christie-Oleza

PROVIDER: PXD072352 | Pride | 2026-01-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20250406_195126_01_sk2_PE_01_95ngul_5ul_Mar_micro_19min_trap150bar_directDIA_PDparam_060425_1629.sne	Other
	20250406_220156_02_sk2_PE_02_70ngul_7ul_Mar_micro_19min_trap150bar_directDIA_PDparam_060425_1630.sne	Other
	20250406_220331_03_sk2_PE_03_57ngul_9ul_Mar_micro_19min_trap150bar_directDIA_PDparam_060425_1631.sne	Other
	20250407_062950_04_sk2_C16_01_68ngul_5ul_Mar_micro_19min_trap150bar_directDIA_PDparam_060425_1632.sne	Other
	20250407_063233_05_sk2_C16_02_38ngul_7ul_Mar_micro_19min_trap150bar_directDIA_PDparam_060425_1633.sne	Other

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Project description:In pregnancies involving preeclampsia (PE), there is evidence that the fetal-placental unit is under oxidative stress. Here we examined primary cell lines generated from umbilical cords (UC) delivered by mothers who had either a normal pregnancy or experienced early onset PE to determine whether the two had distinguishable phenotypes. While all UC provided outgrowths when established in 4 % O2, success was less assured for PE cords under ambient (20 % O2) conditions (P < 0.05). Moreover, proliferation rates of established PE lines, although similar to controls in 4 % O2, were significantly lower in 20 % O2. PE lines grown in 4 % O2 were also more susceptible to the pro-oxidant diethylmaleate than control lines, and unlike controls, were not protected by glutathione. Transcriptome profiling revealed only a few differentially regulated genes between PE lines and controls in 4 % O2 conditions, but confirmed the more severely stressed phenotype of the PE lines under 20 % O2. We conclude that the primary UC cell lines generated from PE births maintain a susceptibility to oxidative stress that is stable over many cell divisions, but whether the basis of this vulnerability is genetic or epigenetic remains unclear. RNA was isolated from early passages (< p5) of UC fibroblast lines (15 PE and 9 CTL lines, grown in T25 flasks) when they reached ~90% confluency in 4 % O2 conditions. In order to collect RNA from cells under 20 % O2, cell lines at either p 4, 5, or 6 were switched from 4 % O2 conditions to 20 % O2 conditions when they were approximately 20 % confluent. When they reached ~90% confluency (generally 2 days in 4 % O2 and 3-4 days in 20 % O2 conditions), medium was removed and RNA STAT60 (I ml; Tel-Test, Friendswood, TX) was immediately added to each T25 flask and RNA extracted by following the manufacturer’s instructions. The samples of RNA were submitted to University Texas Southwestern Medical Center Microarray Core Facility (https://microarray.swmed.edu/) and microarray analysis performed with Illumina HumanHT-12 v4 expression BeadChips. Raw intensity data were background subtracted by using BeadStudio software and analyzed further by GeneSpring 12.6 software (Agilent Technologies Inc., Santa Clara CA), according to the advanced workflow protocol: percentile shift and filter by flags (detected).

Dataset Information

Shotgun proteomic analysis of the marine bacteria Alcanivorax sp. 24 and Alcanivorax borkumensis SK2 grown with weathered polyethylene.

Dataset's files

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