Dataset Information

Nanostructures regulate cell adhesion behavior through mechanotransduction-mediated signaling pathways

ABSTRACT: Nanotopography has a significant regulatory effect on cell adhesion behavior. We fabricated titanium alloy surfaces with titanium dioxide nanotubes using electrochemical anodization, which exhibited increased surface roughness and superhydrophilicity. Using single-cell force spectroscopy, we observed that the nanotubular structure altered the mechanical forces experienced at the cell-surface interface. Proteomic analysis revealed that Nesprin-1 protein transmits these signals to the intracellular core signaling system, leading to the upregulation and activation of GSK3β, STAT3, and related pathways. These events regulate cytoskeletal remodeling and adhesion complex assembly, thereby promoting cell adhesion.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mesenchymal Cell

SUBMITTER: Shawn Shawn

LAB HEAD: Chenao Xiong

PROVIDER: PXD072594 | Pride | 2026-05-25

REPOSITORIES: Pride

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Nanotopography-Mediated Mechanotransduction Enhances hBMSCs Adhesion on TiO<sub>2</sub> Nanotubes.

Xiong Chenao C Feng Hui H Lu Liyang L Jing Zehao Z Wang Youhao Y Yang Yiyuan Y Meng Dexuan D Zhang Yichen Y Li Weishi W Cai Hong H

Journal of functional biomaterials 20260419 4

Titanium and its alloys are widely used for orthopedic implants, but their intrinsic bioinertness may hinder osseointegration. In this study, titanium dioxide nanotube (TNT) arrays were fabricated on Ti-6Al-4V scaffolds via anodization, and their effects on the adhesion behavior of human bone marrow mesenchymal stem cells (hBMSCs) were investigated. Surface characterization showed that anodization successfully generated ordered TNT layers, increased surface roughness, enhanced protein adsorption ...[more]

PMID: 42042306

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Project description:Tissue response following implantation determines the success of the healing process. This response is not only dependent on the chemical properties of the implant surface but also by the surface topography or its roughness. Although in vitro and in vivo studies show improved results with rough- and fluoride-modified implants, the mechanisms behind these findings are still unknown. Here, we have used a two step procedure to identify novel genes related to the early cell response of primary human osteoblasts to roughness and fluoride-modified titanium implants. 217 genes were identified by microarray analysis as response genes to roughness and 198 genes as response genes to fluoride. 11 of these identified genes have been related to bone and mineralization and were further investigated by real-time RT-PCR. After one day of culture, TLR3, ANKH, DCN, OC and RUNX2 were classified as responsive genes to roughness; DLX2 and TUFT1 as responsive genes to fluoride treatment. COLL-I, PTHLH, HES1, FST, ENPP1 and THRA as responsive genes to both, roughness and fluoride treatment. In conclusion, our strategy was useful for identifying novel genes that might be involved in the early response of osteoblasts to roughness and fluoride treatment of titanium implants. Tissue response following implantation determines the success of the healing process. This response is not only dependent on the chemical properties of the implant surface but also by the surface topography or its roughness. Although in vitro and in vivo studies show improved results with rough- and fluoride-modified implants, the mechanisms behind these findings are still unknown. Here, we have used a two step procedure to identify novel genes related to the early cell response of primary human osteoblasts to roughness and fluoride-modified titanium implants. 217 genes were identified by microarray analysis as response genes to roughness and 198 genes as response genes to fluoride. 11 of these identified genes have been related to bone and mineralization and were further investigated by real-time RT-PCR. After one day of culture, TLR3, ANKH, DCN, OC and RUNX2 were classified as responsive genes to roughness; DLX2 and TUFT1 as responsive genes to fluoride treatment. COLL-I, PTHLH, HES1, FST, ENPP1 and THRA as responsive genes to both, roughness and fluoride treatment. In conclusion, our strategy was useful for identifying novel genes that might be involved in the early response of osteoblasts to roughness and fluoride treatment of titanium implants. Human osteoblasts were cultured for 24 hours on titanium disks modified either with polished surfaces (P), grit-blasted surfaces (GB), or grit-blasted High Fluor treated surfaces (GB-HF). After 24 hours of culture RNA was isolated. 3 arrays were hybridized, one with the isolated RNA from cells cultured on titanium polished surfaces (P), one with the isolated RNA from cells cultured on titanium grit-blasted surfaces (GB) and one with the isolated RNA from cells cultured on titanium grit-blasted HF treated surfaces (GB-HF).

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Dataset Information

Nanostructures regulate cell adhesion behavior through mechanotransduction-mediated signaling pathways

Dataset's files

Publications

Nanotopography-Mediated Mechanotransduction Enhances hBMSCs Adhesion on TiO<sub>2</sub> Nanotubes.

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Dataset Information

Nanostructures regulate cell adhesion behavior through mechanotransduction-mediated signaling pathways

Dataset's files

Publications

Nanotopography-Mediated Mechanotransduction Enhances hBMSCs Adhesion on TiO&lt;sub&gt;2&lt;/sub&gt; Nanotubes.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Nanotopography-Mediated Mechanotransduction Enhances hBMSCs Adhesion on TiO<sub>2</sub> Nanotubes.