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Modifications of histone H3.1 and H3.3

ABSTRACT: Nuclei were isolated from leaves of transgenic plants expressing HA-tagged H3.1 (HTR5) or H3.3 (HTR13). MNase treated nuclear extracts were used for immunoprecipitation with HA agarose beads (Roche). Immunoprecipitated proteins were analysed by 4-20% SDS-PAGE, silver stained, and protein bands corresponding to transgenic copy of H3 and endogenous H3 were used for trypsin and LysC digestion followed by LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Richard Imre

LAB HEAD: Frederic Berger

PROVIDER: PXD072852 | Pride | 2026-01-27

REPOSITORIES: Pride

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			Action	DRS
	20150709_QEx6p_RSLC4_Lorkovic_Berger_GMI_50proc_21_lysC.raw	Raw
	20150709_QEx6p_RSLC4_Lorkovic_Berger_GMI_50proc_21_lysC_MSAmanda_Borg-AT-histone-db_deamidR_methyl-research.msf	Msf
	20150709_QEx6p_RSLC4_Lorkovic_Berger_GMI_50proc_21_try.raw	Raw
	20150709_QEx6p_RSLC4_Lorkovic_Berger_GMI_50proc_21_try_MSAmanda_Borg-AT-histone-db_deamidR_methyl-research.msf	Msf
	20150709_QEx6p_RSLC4_Lorkovic_Berger_GMI_50proc_22_lysC.raw	Raw

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