Dataset Information

Tenebrio molitor protein mix after growth on Cupravidus necator biomass and oat flakes

ABSTRACT: This study assesses the crude protein mix from whole mealworms as an alternative to the traditionally used chlorinated organic solvents for the extraction of the greenest biodegradable biopolymers emerging on the market - polyhydroxyalkanoates. The biopolymers recovered were of comparable quality to those recovered by CHCl3 extraction, as determined by GPC and NMR. Crude protein mix exceeded the efficiency of commercial enzymes at up to 10-fold lower enzyme load. Therefore, this approach is not only a safer, non-toxic alternative to traditional organic solvents, but also an economically viable option. A proteomic study of the mealworm proteins after growth on 100% bran (TM mix control) and on 100% Cupravidus necator biomass (TM mix 2) was performed to identify enzymes responsible for the recovery effect.

INSTRUMENT(S):

ORGANISM(S): Tenebrio Molitor

TISSUE(S): Whole Body

SUBMITTER: Romanos Siaperas

LAB HEAD: Jasmina Nikodinovic-Runic

PROVIDER: PXD072887 | Pride | 2026-05-04

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 46

Publications

Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates.

Bogojevic Sanja Skaro SS Pantelic Brana B Ponjavic Marijana M Ilic-Tomic Tatjana T Milivojevic Dusan D Guzik Maciej M Rychwalski Marcin M Siaperas Romanos R Topakas Evangelos E Carvalheira Mónica M Freitas Filomena F Concórdio-Reis Patrícia P Nikodinovic-Runic Jasmina J

Microbial biotechnology 20260501 5

Polyhydroxyalkanoates (PHAs) are intracellular microbial polyesters whose commercial deployment is strongly influenced by downstream processing costs and environmental burden. Here, we demonstrate a nearly solvent-free, mild aqueous strategy for the recovery of PHAs directly from wet bacterial biomass using crude enzyme systems derived from Tenebrio molitor. Under optimized conditions (0.6 wt% crude protein, pH 7.6, 40°C), near-quantitative (≥ 95%) recovery of PHB and PHB/HV and up to 60% recove ...[more]

PMID: 42059372

Similar Datasets

Project description:Various environmental bacteria were adapted to the presence of toxic and recalcitrant organic solvents. Cupriavidus metallidurans CH34, a β-proteobacterium that was found in industrial environments is known as a bacterial model to study heavy metal resistance. Interestingly, genome screening of CH34 also reveals the existence of genes involved in the degradation of recalcitrant organic solvents, such acetone and aromatic compounds. Here, we showed that this bacterium could resist a large variety of organic solvents, and was also able to metabolize some of them. In particular, investigations were focused on acetone and isopropanol catabolism. Integrative studies based on transcriptomic (DNA microarrays), proteomic (2D-DIGE and Isotope-Coded Protein Label technology) and biochemical analyses (enzyme purification and characterization) showed a similar catabolic pathway for both molecules which involved the AcxABC acetone carboxylase. First, isopropanol is oxidized into acetone by the Adh alcohol dehydrogenase. Acetoacetate production from acetone is then catalyzed by the acetone carboxylase. The generated acetoacetate molecules are then transformed by the PacIJ 3-oxoacid CoA-transferase, to acetoacetyl-CoA and succinate. Finally, an acetyl-CoA acetyltransferase catalyses the hydrolysis of acetoacetyl-CoA into 2 acetyl-CoA that are introduced into the glyoxylate cycle. As demonstrated, key enzymes of the acetone/isopropanol catabolism (encoded by acx and ald genes) are under the control of a σ54-dependent RNA polymerase. Moreover, the catabolic pathway involved in acetone and isopropanol consumption was repressed when gluconate was given as alternative carbon substrate, displaying a new example of diauxie. The results presented here provide a comprehensive picture of acetone and isopropanol biodegradation in Cupriavidus metallidurans CH34 and strongly support the fact that CH34 can be considered as a solvent tolerant bacterium. Two-condition experiments. Comparing samples after induction with acetone versus non-induced samples. Biological triplicate. Each array contains 3 technical replicates.

			Action	DRS
	BB_r1_1.mzML.quant	Mzml
	BB_r1_1.raw	Raw
	BB_r1_2.mzML.quant	Mzml
	BB_r1_2.raw	Raw
	BB_r1_3.mzML.quant	Mzml