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Characterization of an animal polyhydroxyalkanoate depolymerase that degrades microbial PHAs

ABSTRACT: Microbial species produce the storage biopolymer polyhydroxyalkanoate (sPHA), as a carbon and energy source. However, only bacteria and fungi are currently known to degrade microbial sPHAs, using an enzyme called PHA depolymerase (PHAD). This study shows that animals also have a PHAD that can degrade sPHA. We discovered a PHAD in the gutless oligochaete, Olavius algarvensis. These marine worms gain their nutrition by digesting their bacterial symbionts, with sPHA making up to 42% of the carbon in their dominant symbiont. In gutless oligochaetes, this sPHA-synthesizing symbiont is present in the post-genital trunk regions and absent in the head region of the worm (Ott et al., Springer 2023). Based on this previous observation, the worms were dissected to remove the head portion (“tip”) from the body (“trunk”) just above the genital pad region. Samples consisted of single worms and pools of three or five worms washed in 99% ethanol prior to dissection (n = 3 for single and pooled samples). In addition, one sample set consisted of single worms not washed with ethanol (n = 3). Dissected samples were placed in 2 ml cryo-vials, flash frozen in liquid nitrogen, and stored at -80 °C prior to protein digestion. The whole sample set was processed for proteomics.

INSTRUMENT(S):

ORGANISM(S): Olavius Algarvensis Gamma 3 Endosymbiont Olavius Algarvensis Olavius Algarvensis Gamma 1 Endosymbiont Candidatus Thiosymbion Olavius Algarvensis Delta 4 Endosymbiont Olavius Algarvensis Spirochete Endosymbiont Olavius Algarvensis Delta 1 Endosymbiont

SUBMITTER: Grace D'Angelo

LAB HEAD: Manuel Liebeke

PROVIDER: PXD072931 | Pride | 2026-07-28

REPOSITORIES: Pride

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			Action	DRS
	20230829_MS128_APO_OalgTipvTrunk_A1_04.msf	Msf
	20230829_MS128_APO_OalgTipvTrunk_A1_04.raw	Raw
	20230829_MS128_APO_OalgTipvTrunk_A2_50.msf	Msf
	20230829_MS128_APO_OalgTipvTrunk_A2_50.raw	Raw
	20230829_MS128_APO_OalgTipvTrunk_B1_12.msf	Msf

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Characterization of an animal polyhydroxyalkanoate depolymerase that degrades microbial PHAs

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