Dataset Information

ULK1-Driven Autophagy Modulation Alters Tumor-Promoting Pathways in Triple-Negative Breast Cancer

ABSTRACT: This dataset supports a study investigating the proteomic consequences of pharmacological ULK1 modulation in triple-negative breast cancer. MDA-MB-231 cells were treated with the ULK1 activator LYN-1604 or the ULK1 inhibitor MRT68921 at EC50 concentrations (29.8 µM and 2.6 nM, respectively) for 24 h. Autophagic activity was evaluated by LC3 immunoblotting and fluorescence microscopy, and quantitative label-free LC–MS/MS was used to characterize global protein abundance changes following ULK1 activation versus inhibition. Differentially regulated proteins were further interpreted using interaction network analysis and pathway enrichment approaches to identify convergent tumor-related and immune-associated signatures linked to ULK1 perturbation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Breast Cancer

SUBMITTER: Merve Gülsen Bal Albayrak

LAB HEAD: Merve Gulsen Bal Albayrak

PROVIDER: PXD072955 | Pride | 2026-03-09

REPOSITORIES: Pride

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Publications

ULK1-driven autophagy modulation alters tumor-promoting pathways in triple-negative breast cancer.

Bal Albayrak Merve Gulsen MG Yanar Sevinc S Korak Tuğcan T Akpinar Gurler G Kasap Murat M

Medical oncology (Northwood, London, England) 20260303 4

Triple-negative breast cancer (TNBC) is an aggressive subtype with limited targeted therapies and poor prognosis. Unc-51-like kinase 1 (ULK1), a central regulator of autophagy, has emerged as a potential therapeutic node in cancer but remains poorly understood in TNBC. Here, we investigated the proteomic consequences of pharmacological ULK1 modulation in MDA-MB-231 TNBC cells. Cells were treated with the ULK1 activator LYN-1604 or inhibitor MRT68921 at EC₅₀ concentrations. Autophagic activity, v ...[more]

PMID: 41774346

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			Action	DRS
	CONTROL.raw	Raw
	CONTROL2.raw	Raw
	CONTROL3.raw	Raw
	LYN.fasta	Fasta
	LYN.mzTab	Mztab