Proteomics

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Treatment of human oocytes with extracellular vesicles from follicular fluid during rescue in vitro maturation enhances maturation rates and modulates oocyte proteome and ultrastructure

ABSTRACT: This project aimed to elucidate whether human follicular fluid–derived extracellular vesicles (ffEVs) enhance oocyte maturation during rescue in vitro maturation (rIVM). We characterised the protein cargo of ffEVs isolated from follicles containing oocytes at different maturation stages (GV, GVBD, and MII), identifying distinct proteomic signatures associated with follicular competence and oocyte maturity. Subsequently, single-cell proteomic profiling of IVM-matured oocytes supplemented with ffEVs demonstrated that ffEV treatment induces significant changes in oocyte protein abundance, including factors linked to oocyte maturation, endoplasmic reticulum function, steroid biosynthesis, and cytoskeletal organisation. These proteomic alterations were accompanied by changes in oocyte ultrastructure and organelle architecture.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Oocyte, Follicular Fluid

SUBMITTER: Mara Saenz-de-Juano  

LAB HEAD: Prof. Dr. Susanne Ulbrich

PROVIDER: PXD073018 | Pride | 2026-04-06

REPOSITORIES: Pride

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20221214_C30256_002_S434879_256ESH_MII.raw Raw
20221214_C30256_003_S434883_170ANM_MII.raw Raw
20221214_C30256_004_S434886_129ASK_MII.raw Raw
20221214_C30256_005_S434888_296CLA_MII.raw Raw
20221214_C30256_006_S434890_39JAK_GVBD.raw Raw
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Treatment of human oocytes with extracellular vesicles from follicular fluid during rescue <i>in vitro</i> maturation enhances maturation rates and modulates oocyte proteome and ultrastructure.

Makieva Sofia S   Saenz-de-Juano Mara D MD   Almiñana Carmen C   Bauersachs Stefan S   Bernal-Ulloa Sandra S   Xie Min M   Velasco Ana G AG   Cervantes Natalia N   Sachs Maike M   Ulbrich Susanne E SE   Leeners Brigitte B  

Human reproduction open 20260309 2

<h4>Study question</h4>Could follicular fluid-derived extracellular vesicles (ffEVs) benefit human oocyte rescue <i>in vitro</i> maturation (rIVM)?<h4>Summary answer</h4>Supplementation of rIVM culture with ffEVs isolated from mature follicles enhanced oocyte maturation rates by >20%, inducing changes in oocyte protein profile and organelle distribution.<h4>What is known already</h4>IVM involves the culture of immature germinal vesicle (GV) oocytes under set laboratory conditions to allow for th  ...[more]

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