Proteomics

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Proteomic Analysis of Ginkgolic Acid Targets in HCCLM3 Cells via DARTS-LC-MS/MS

ABSTRACT: This study aims to identify the potential protein targets of Ginkgolic Acid in human hepatocellular carcinoma HCCLM3 cells using the Drug Affinity Responsive Target Stability (DARTS) assay combined with LC-MS/MS proteomics. Whole cell lysates from Ginkgolic Acid-treated and vehicle control (DMSO) HCCLM3 cells were subjected to limited proteolysis with proteinase K. The digested protein samples were separated by SDS-PAGE, and differential protein bands were excised, digested in-gel, and analyzed by mass spectrometry. The raw data will be used to identify proteins whose stability is altered upon Ginkgolic Acid binding, providing insights into its anti-tumor mechanisms.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Huizhen Zou  

LAB HEAD: shuguo zheng

PROVIDER: PXD073111 | Pride | 2026-03-30

REPOSITORIES: Pride

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Action DRS
480_FPEP240062611-1A.msf Msf
480_FPEP240062611-1A.pdResult Other
480_FPEP240062611-1A.raw Raw
480_FPEP240062612-1A.msf Msf
480_FPEP240062612-1A.pdResult Other
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Publications

Ginkgolic acid targets HSPA8 to trigger ferroptosis in hepatocellular carcinoma <i>via</i> chaperone-mediated autophagy-dependent GPX4 degradation.

Liu Ruohong R   Zhang Yufan Y   Zou Huizhen H   Zhang Min M   Yang Zhiyuan Z   Liao Kexi K   Wang Guoping G   Liu Beiyu B   Tang Lijun L   Kang Xia X   Zheng Shuguo S  

Pharmaceutical biology 20260325 1

<h4>Context</h4>Ginkgolic acid (GAC), one of the major active constituents of <i>Ginkgo biloba L. (Ginkgoaceae)</i> extract, has been reported as a potential anticancer agent.<h4>Objective</h4>To investigate the effect of GAC on the viability of human hepatocellular carcinoma (HCC) cells and to identify its primary target and underlying mechanism.<h4>Materials and methods</h4>Human HCC cell lines and an orthotopic HCC mouse model were employed. The mechanism of GAC was elucidated through integra  ...[more]

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