Proteomics

Dataset Information

0

Systematic cysteine scanning identifies a druggable pocket in oncogenic KRAS


ABSTRACT: We developed Cysteine Mapping of Accessible Pockets (CysMAP), a method for identifying druggable pockets in proteins. CysMAP employs systematic pooled cysteine variant libraries screened against diverse covalent compound libraries by intact LC-MS. Here, we applied CysMAP to 189 KRAS(G12D) variants, purifying KRAS Cys-variants and screening them against 47 covalent compounds, quantifying accessibility and reactivity across KRAS(G12D).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: L. Moore van Tienen  

LAB HEAD: William R

PROVIDER: PXD073201 | Pride | 2026-02-05

REPOSITORIES: Pride

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