Systematic cysteine scanning identifies a druggable pocket in oncogenic KRAS
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ABSTRACT: We developed Cysteine Mapping of Accessible Pockets (CysMAP), a method for identifying druggable pockets in proteins. CysMAP employs systematic pooled cysteine variant libraries screened against diverse covalent compound libraries by intact LC-MS. Here, we applied CysMAP to 189 KRAS(G12D) variants, purifying KRAS Cys-variants and screening them against 47 covalent compounds, quantifying accessibility and reactivity across KRAS(G12D).
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
SUBMITTER:
L. Moore van Tienen
LAB HEAD: William R
PROVIDER: PXD073201 | Pride | 2026-02-05
REPOSITORIES: Pride
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