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Taxifolin protects HaCaT keratinocytes from UVB damage

ABSTRACT: The photoprotective efficacy and underlying mechanisms of taxifolin against UVB-induced injury were evaluated in human immortalized keratinocytes (HaCaT).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yu-Pei Chen

LAB HEAD: Yu-Pei Chen

PROVIDER: PXD073279 | Pride | 2026-04-06

REPOSITORIES: Pride

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			Action	DRS
	Ig01CYP_C_1_1.raw	Raw
	Ig01CYP_C_2_2.raw	Raw
	Ig01CYP_C_3_3.raw	Raw
	Ig01CYP_Tax_1_7.raw	Raw
	Ig01CYP_Tax_2_8.raw	Raw

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Publications

Multi-Target Photoprotection by Taxifolin Against UVB-Induced Keratinocyte Injury Through UVB Filtration, ROS Scavenging and Transcriptomic-Proteomic Reprogramming.

Chen Fangfang F Cai Yihan Y Wu Jinxiong J Fang Nengzhen N Li Fei F Wu Hongtan H Chen Yu-Pei YP

Biomolecules 20260304 3

Taxifolin, a natural flavonoid, consistently exerts cytoprotective effects against various oxidative stresses. In this study, we systematically evaluated its photoprotective efficacy and underlying mechanisms against ultraviolet B (UVB)-induced injury in human immortalized keratinocytes (HaCaT). Cell viability and apoptosis were assessed by MTT, fluorescence staining, and flow cytometry, while integrative transcriptomic and proteomic analyses were employed to identify core pathways and key media ...[more]

PMID: 41897323

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Aloin Protects Against UVB-Induced Apoptosis

Project description:This study comprehensively investigates the photoprotective effects of aloin against UVB-induced damage in HaCaT cells, elucidating its antioxidant capacity and its role in preventing cellular apoptosis. The proteomic effect of 50 μg/mL aloin on HaCaT cell damage induced by UVB was evaluated through comparative protein expression. Compared to control cells, UVB irradiation significantly altered protein expression in HaCaT cells, with 64 proteins upregulated and 236 proteins downregulated. In cells treated with both UVB and aloin, 79 proteins were upregulated and 84 proteins were downregulated compared to the UVB-only group. Proteomic analysis showed that aloin modulated the expression of proteins involved in critical signaling pathways, including PI3K-Akt, p53, TGF-β and pathways in cancer, promoting cell survival. Aloin upregulated proteins associated with cell cycle regula-tion and antioxidant responses, such as CCND3, GSTM4, GNA12, SKIL, YWHAZ, and PKN3 while downregulating pro-apoptotic protein FOXO3.

2025-08-04 | PXD060808 | Pride

Characterization of cyclobutane pyrimidine dimer-dependent modulation of gene expression in human keratinocytes following UVB exposure

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Studying Gene Expression Profiles of UV-Irradiated Human Keratinocytes by cDNA Microarray

Project description:The goal of this thesis is to study the response of a human keratinocyte cell line (HaCaT cells) after UVB irradiation. In order to develop a system for UV irradiation, we firtstly studied the UV illumination system to find out a stable and reliable condition for UV irradiation experiments. Further, we found out the proper dose of UVB radiation and time points after UVB irradiation by performing MTT assay and trypan blue viability test. According to the results of MTT assay and trypan blue viability test, the cellular activity as well as survival rate of UVB-irradiated cells were in proportion to the radiation doses within a lower UVB dose range. We wanted to find out the possible reasons for this phenomenon by using cDNA microarray. We hope the thesis could be a guide for the following researches, and be useful in the clinical studies about the UV damage. Keywords: dose response and time course Loop-designed microarray experiments were performed in our study. Twenty-one samples are arranges as seven small loops and one large loop, with three and seven microarray slides for each small and large loop, respectively. This set of loop-designed microarray experiment contains one control untreated (non UVB-treated) samples and two UVB-treated samples (one low dose and one high dose UVB-treated) at each time point (total seven time points).

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Study of transcriptional effects of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line.

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