ZNFX1 activity-based probe crosslinking mass spectrometry

ABSTRACT: The aim of this experiment was to identify the catalytic cysteine in the antiviral E3 ligase ZNFX1 using activity-based probe (ABP)–enabled cross-linking mass spectrometry (XL-MS). A biotinylated E2~ubiquitin activity-based probe containing a cysteine-electrophile trap was used to covalently capture ubiquitin a transfer intermediate between UBE2D2~Ub and full-length human ZNFX1. Cross-linked peptide mapping was performed to identify probe-labelled residues and putative catalytic site.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Satpal Virdee  

LAB HEAD: Satpal Virdee

PROVIDER: PXD073419 | Pride | 2026-05-22

REPOSITORIES: Pride