Dataset Information

Interactome of ASXL1 via TAP-MS proteomics

ABSTRACT: Functional caracterization and mapping of ASXL1-specific ptotein-protein interactions

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Blood Cell, Permanent Cell Line Cell

DISEASE(S): Chronic Myeloid Leukemia

SUBMITTER: El Bachir AFFAR

LAB HEAD: El Bachir AFFAR

PROVIDER: PXD073614 | Pride | 2026-03-30

REPOSITORIES: Pride

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Publications

Recruitment of BRD4 to the ASXL1 genomic targets depends on the extra-terminal domain of BRD4.

Selvam Karthik K Lu Shuang S Messmer Clémence C Pang Yakun Y Biswas Soumi S Khalil Moustafa M Zhang Peng P Tulaiha Rima R Zhou Ming-Ming MM Kitamura Toshio T Lauberth Shannon M SM Blanco M Andres MA Yang Feng-Chun FC Affar El Bachir EB Zhao Zibo Z Zeng Lei L Wang Lu L Kutateladze Tatiana G TG

Nature communications 20260217 1

ASXL1 is a well-established driver of a wide range of cancers. Here, we identify a high level of genetic correlation between ASXL1 and the major transcriptional activator BRD4 in cancer cells and characterize the molecular mechanism underlying this correlation. Structural and biochemical data show the formation of a tight complex between the extraterminal domain of BRD4 and the BRD4-binding motif of ASXL1. ChIP-seq analysis of mutated ASXL1 that cannot bind BRD4 demonstrates that the recruitment ...[more]

PMID: 41702923

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