Dataset Information

Proteomics Identification of Opisthorchis viverrini Antigens in Excretory-Secretory matrix

ABSTRACT: Opisthorchis viverrini (Ov), a Group I biological carcinogen classified by the WHO, is a leading cause of cholangiocarcinoma (CCA) in the Greater Mekong Subregion. Transmission occurs via consumption of raw or undercooked fish harboring Ov metacercariae, which mature into adult Ov worms in the biliary tract, triggering chronic inflammation and malignant transformation. Despite the widespread use of Ov-antigen rapid test kits (Ov-ATKs) for non-invasive screening, current diagnostics are limited by cross-reactivity and dependence on crude Ov antigens. In this study, we employed a label-free proteomics approach to comprehensively analyze infection-associated stages of Ov (metacercariae and adult worms) to compare with urinary sample from Ov-infected and CCA patients. A total of 17,287 proteins were identified, 2,768 protein shares across group of samples and 3,977 proteins were consistently upregulated in Ov-positive urine samples, we provide the first direct evidence that Ov-derived proteins are present in the urine of infected individuals, confirming systemic dissemination from the parasite. We also identified key protein signatures associated with both acute and chronic infection. These findings offer key insights into parasite specific protein and support the development of recombinant antigen-based diagnostics, enhancing Ov-ATKs specificity and enabling more accurate, scalable screening in endemic areas.

INSTRUMENT(S):

ORGANISM(S): Opisthorchiidae

TISSUE(S): Urine

SUBMITTER: Chuthamat Klanghon

LAB HEAD: Integrative Omics Medicine & Synthetic Biology Research Group

PROVIDER: PXD073886 | Pride | 2026-02-26

REPOSITORIES: Pride

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Source:

			Action	DRS
	OV-Adult-1_RA4_01_10238.d.zip	Other
	OV-Adult-2_RA4_01_10243.d.zip	Other
	OV-Adult-3_RA4_01_10248.d.zip	Other
	OV-Ag-CCA-1_RA6_01_10240.d.zip	Other
	OV-Ag-CCA-2_RA6_01_10245.d.zip	Other

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Project description:Epithelial ovarian carcinoma (EOC) is an aggressive tumor often diagnosed at an advanced stage, when there is little prospect for cure. Despite some advances in surgical and chemotherapeutic strategies, only marginal improvements in patient outcome have been obtained. Hence, understanding the biological mechanisms underpinning EOC progression is critical for its treatment and to ameliorate patients survival. Recently, we reported that CD157 is expressed in EOC and controls tumor cell migration and invasion. Using stable overexpression and knockdown in OVCAR-3 and OV-90 ovarian cancer cell lines, we demonstrated that CD157 overexpression promotes morphological and functional changes, characterized by downregulation of epithelial marker and upregulation of mesenchymal ones. These are mediated at the transcriptional level by altering the expression of Snail and Zeb1 transcriptional repressors. The effects of CD157 overexpression on ovarian cancer phenotype translate into increased tumor cell motility and mesotelial invasion, while its knockdown significantly reduces the migratory potential, implying a direct correlation between CD157 expression levels and EOC aggressiveness. The analysis of the transcriptomic profiling highlighted 378 significantly differentially expressed genes, representing the signature of CD157-overexpressing EOC cells. The overall picture deduced from the analysis of these modulated transcripts indicated that high CD157 expression results in strengthening of a number of biological functions that favour tumor progression (including cell differentiation, cell motility and migration), and weakening of selected biological processes that hinder the tumor progression (such as apoptosis, cell death and response to stress). Collectively, these data support a causal role of CD157 in the control of ovarian cancer progression motivating the existence of a direct correlation between the expression levels of CD157 and the adverse clinical outcome in EOC patients, and suggest that CD157 may represent a valuable therapeutic target. Gene expression analysisi of control cell lines (OVCAR-3/mock and OV-90/mock) and testing cell lines (OVCAR-3/CD157 and OV-90/CD157), with two replicates, with dye swap, performed for each sample.

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Proteomics Identification of Opisthorchis viverrini Antigens in Excretory-Secretory matrix

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