Dataset Information

A Proteomic Profiling of Tegumental Washes in Ligula intestinalis (Cestoda) and Host Cavity Washes of Abramis brama (Leuciscidae)

ABSTRACT: The aim of this study was to characterize the molecular interactions zone between the plerocercoid of Ligula intestinalis and its host, a common bream Abramis brama. Using HPLC-MS/MS we performed a comparative proteomic analysis of washes from the parasite surface and from the body cavity of infected and uninfected fish. We identified both parasitic and host proteins on the helminth surface, reflecting an active molecular crosstalk. Functional analysis revealed cestode-specific metabolic and signaling pathways (eg. such as proline biosynthesis and TOR signaling), including those for amino acid biosynthesis, lipid transport, and nutrient sensing. Within the body cavity of infected fish, we detected parasite-derived proteins such as immunomodulators, proteases, and metabolic enzymes. Quantitative changes in host wash proteomes indicated activation of humoral immunity alongside remodeling of proteolytic regulation. Collectively, these data reveal the molecular foundation of L. intestinalis adaptation and the dynamic host-parasite relationships in this system.

INSTRUMENT(S):

ORGANISM(S): Abramis Brama Ligula Intestinalis

SUBMITTER: Albina Tsekova

LAB HEAD: Tsekova Albina

PROVIDER: PXD074238 | Pride | 2026-07-10

REPOSITORIES: pride

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			Action	DRS
	0425_SF_10_DDA_RB2_1_10073.d.zip	Other
	0425_SF_10_DDA_RB2_1_10073_5.3.236.mgf	Mgf
	0425_SF_10_DDA_RB2_2_10074.d.zip	Other
	0425_SF_10_DDA_RB2_2_10074_5.3.236.mgf	Mgf
	0425_SF_11_DDA_RB3_1_10077.d.zip	Other

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Project description:The liver fluke Fasciola hepatica is an economically important pathogen of livestock. Fasciolosis in humans is an important re-emerging zoonosis, with 180 million people at risk. Development of novel control strategies requires an understanding of parasite virulence and tissue invasion, and of how the parasite evades and modulates the immune response during early infection. A combination of immunological and proteomic analyses was employed to investigate the peritoneal fluid of sheep infected with F. hepatica to characterise early tissue invasion and liver pathogenesis. At 18 days post-infection (dpi), histopathology of sheep liver showed white necrotic foci/tracts indicative of F. hepatica migration. Within the peritoneal fluid of infected animals, specific F. hepatica antigen FhCL1 antibodies coincided with an intense innate and adaptive cellular immune response, with increasing numbers of total leukocytes and a marked eosinophilia (49%). Cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated but not statistically significant at 18 dpi compared to uninfected sheep indicating that whilst the immune response is developing it has not polarised to the Th2 type associated with chronic fasciolosis. Proteomic analysis of the peritoneal fluid identified 178 proteins, with 25 proteins more highly expressed in the infected animals, based on fold changes in protein concentration compared to the uninfected animals. Components of the liver extracellular matrix, including collagen, periostin and the adhesion protein, VCAM-1, exhibited increased expression associated with early fasciolosis, which may be important in signalling host immune responses to tissue damage. In early F. hepatica infection cellular infiltration with marked eosinophilia, adaptive immune responses and products of liver pathology are evident in the peritoneum. Although cytokine responses are developing they are mixed and not yet polarised to a Th2-type. We have characterised biomarkers of parasite-induced liver damage which could be exploited for diagnosis, and vaccine development aimed at reducing/preventing liver pathology.

Dataset Information

A Proteomic Profiling of Tegumental Washes in Ligula intestinalis (Cestoda) and Host Cavity Washes of Abramis brama (Leuciscidae)

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