Dataset Information

Effect of cancer-associated fibroblast exosomes on murine T cells

ABSTRACT: We profiled how cancer associated fibroblast exosomes alter T cells in mouse breast tumor setting using mass spectrometry. Briefly, exosomes were isolated from breast cancer associated fibroblasts from the MCa-P1362 cell line and then applied to primary CD8+ T cells from BALB/c mice. After exposure for 24 hours, T cells stimulated with anti-CD3/CD28 antibodies for indicated times and were harvested and subjected to quantitative proteomic and phosphoproteomic analysis by mass spectrometry to capture changes in protein expression and signaling. Unstimulated cells and stimulated cells not exposed to exosomes were used as comparators.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Carl White

LAB HEAD: Dennis Jones

PROVIDER: PXD074262 | Pride | 2026-03-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210412_ML_Jones_TCell_Prot_F10_Split_1_164327.raw	Raw
	20210412_ML_Jones_TCell_Prot_F10_Split_2_164610.raw	Raw
	20210412_ML_Jones_TCell_Prot_F10_Split_3_164809.raw	Raw
	20210412_ML_Jones_TCell_Prot_F11_Split_1_164957.raw	Raw
	20210412_ML_Jones_TCell_Prot_F11_Split_2_165238.raw	Raw

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Publications

Collagen-Bearing Exosomes from Breast Cancer-Associated Fibroblasts Promote T-cell Dysfunction.

Jana Samir S Lawton Matthew M Lei Pin-Ji PJ Hui Harold H Emili Andrew A Jones Dennis D

Cancer research communications 20260301 3

Cancer-associated fibroblasts (CAF), a major component of the breast tumor microenvironment, drive immune evasion in various cancers by promoting T-cell exclusion and dampening T-cell activation. Previous studies have implicated CAF-derived soluble factors in mediating these immunosuppressive effects. In this study, we investigated whether exosomes secreted by CAFs could suppress T-cell activity. Inhibition of global exosome secretion in breast tumor-bearing mice significantly reduced tumor grow ...[more]

PMID: 41709670

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