Proteomic Analysis of the Protein Composition of Migrasomes Enriched by Magnetic Aptamer Probe System and Density Gradient Centrifugation

ABSTRACT: This study aims to evaluate the performance of the magnetic aptamer probe (MAP) system in the enrichment of migrasomes. First, the MAP system was constructed by functionalizing biotin-labeled migrasome-targeting aptamers (Apt_B3) onto the surface of NeutrAvidin-coated magnetic sub-micrometre particles. To gain a deeper understanding of the system’s efficacy, we collected 4 clinical plasma samples and isolated the migrasomes using both traditional density gradient centrifugation (DGC) and the MAP system respectively. Subsequently, the protein composition of the migrasomes isolated by these two methods was analyzed using quantitative proteomics. To directly evaluate the reproducibility of our MAP isolation protocol, we compared the proteomic profiles of migrasomes isolated in two independent preparation batches. Batch-1 consisted of two replicates (MAP_a and MAP_b), and Batch-2 comprised four replicates (MAP_1-4). This approach seeks to demonstrate the promising applicability of the MAP system in studies related to clinical disease diagnosis of migrasomes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Extracellular Vesicle

SUBMITTER: Yixin Xu  

LAB HEAD: Yixin Xu

PROVIDER: PXD074521 | Pride | 2026-04-27

REPOSITORIES: Pride