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2-arachidonoylglycerol (2-AG) modulates the proteome and genes associated with the intestinal barrier of Caco-2 cells over the inflammatory response of IL-1β

ABSTRACT: Traumatic brain injury (TBI) is a major risk factor for late-onset neurodegenerative disorders, including Alzheimer’s disease (AD). We investigated whether mild TBI (mTBI) at the presymptomatic stage differentially affects the gut microbiota–endocannabinoidome (eCBome) axis. Caco-2 cells were treated as follows: (i) untreated (CTRL), (ii) IL-1β (10 ng/ml for 24 h), (iii) 2-AG 10 μM (24 h), (iv) 2-AG 10 μM + IL-1β (30 min 2-AG IL-1β 24 h). We characterized proteome-wide quantitative changes in this cell model under different treatment conditions using the LFQ proteomics approach. Our findings identify the gut microbiota–endocannabinoidome axis as a key mediator of long-term outcomes after brain trauma.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Michele Costanzo

LAB HEAD: Michele Costanzo

PROVIDER: PXD074580 | Pride | 2026-07-31

REPOSITORIES: Pride

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			Action	DRS
	2AG_1_1.raw	Raw
	2AG_1_2.raw	Raw
	2AG_2_1.raw	Raw
	2AG_2_2.raw	Raw
	2AG_3_1.raw	Raw

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