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Proteomic profiling to uncover developmental dynamics of autophagy in the cortex

ABSTRACT: Autophagy is an evolutionarily conserved degradation and recycling process through which cells deliver cytoplasmic components such as toxic or defective proteins and organelles to lysosomes for clearance. Unlike dividing cells, neurons depend on degradative pathways to prevent the build-up of cellular waste and to sustain nutrient and energy homeostasis. Emerging evidence indicates that autophagy is particularly critical during early development when neuronal circuits are being established, synaptic connections refined, and activity-dependent mechanisms sculpt overall network architecture. Accordingly, loss of key autophagy-related genes in newly formed neurons disrupts differentiation, synaptic formation and neurotransmission. Despite these insights, the developmental regulation of autophagy genes remains poorly understood, and the composition of the autophagic machinery at synapses is still largely unresolved. To address this, we examined the autophagy-associated proteome within synaptosomes to better understand how autophagic proteins contribute to synaptic processes during critical stages of network formation. Along with transcriptomics data, we provide a foundation for identifying therapeutic targets for neurological disorders linked to impaired synaptic and cellular homeostasis.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cerebral Cortex

SUBMITTER: Annapaola Andolfo

LAB HEAD: Palmieri Michela

PROVIDER: PXD074614 | Pride | 2026-04-03

REPOSITORIES: Pride

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			Action	DRS
	F011697.mzid	Mzid
	F011698.mzid	Mzid
	F011699.mzid	Mzid
	F011700.mzid	Mzid
	F011701.mzid	Mzid

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