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Proteomics of long-term acclimation of the desert cyanobacterium Chroococcidiopsis sp. CCMEE 029 in perchlorate-rich medium and its implications for in situ resource utilization on Mars

ABSTRACT: Space exploration demands sustainable technologies to minimize reliance on Earth-based resources. The extreme-tolerant cyanobacterium Chroococcidiopsis sp. CCMEE 029 exhibits remarkable resistance to perchlorate salts ubiquitously found on Martian soil, holding promise for in situ resource utilization. Exploring the proteomic responses to this chaotropic agent is fundamental for understanding the mechanisms of salt-stress response in cyanobacteria and holds promise to develop biotechnologies based on local resources to support human outposts. Hence, using liquid chromatography–tandem mass spectrometry, we analyzed the protein expression after 21 days of cultivation in the presence of increasing perchlorate concentrations with triplicates per experimental condition (|log2FC| ≥ 0.5; FDR ≤ 0.05). By providing molecular insights into cyanobacterial stress responses, this research advances our understanding of microbial adaptation to environmental constraints occurring on Mars and lays the groundwork for optimizing microbial biotechnologies to support astronauts in long-term missions on the red planet.

INSTRUMENT(S):

ORGANISM(S): Chroococcidiopsis Sp. Ccmee 29

SUBMITTER: Gabriele Rigano

LAB HEAD: Daniela Billi

PROVIDER: PXD074715 | Pride | 2026-03-23

REPOSITORIES: Pride

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			Action	DRS
	Astro_UniRo_12a_7-26-2023_2401.d.zip	Other
	Astro_UniRo_12b_7-26-2023_2405.d.zip	Other
	Astro_UniRo_12c_7-26-2023_2409.d.zip	Other
	Astro_UniRo_24a_7-26-2023_2402.d.zip	Other
	Astro_UniRo_24b_7-26-2023_2406.d.zip	Other

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Dataset Information

Proteomics of long-term acclimation of the desert cyanobacterium Chroococcidiopsis sp. CCMEE 029 in perchlorate-rich medium and its implications for in situ resource utilization on Mars

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