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PCP-MS analysis of insulin resistance in C2C12 myotubes with or without acute insulin stimulation

ABSTRACT: To study dynamic remodelling of protein complexes, C2C12 myotubes were treated with 0.5 mM palmitic acid for 24 h to induce insulin resistance (IR) and then stimulated with or without acute 100 nM insulin for 30 min followed by PCP-MS(n=3 each. Samples were lysed in non-denaturing buffer, separated by BN-PAGE, and each lane cut into 30 x 1.5mm fractions. Each fraction underwent in-gel digestion and was analysed with liquid chromatography – tandem mass spectrometry (LC-MS/MS) using data-independent acquisition (DIA) resulting in the analysis of 360 individual runs.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Benjamin Parker

LAB HEAD: Benjamin Parker

PROVIDER: PXD075147 | Pride | 2026-05-29

REPOSITORIES: Pride

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			Action	DRS
	231224_Acend_BP-7811_P1_A1.raw	Raw
	231224_Acend_BP-7811_P1_A10.raw	Raw
	231224_Acend_BP-7811_P1_A11.raw	Raw
	231224_Acend_BP-7811_P1_A12.raw	Raw
	231224_Acend_BP-7811_P1_A2.raw	Raw

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