Exploratory quantitative proteomic analysis of NMN-treated and TMEV-infected Neuro-2a and RAW264.7 cells

ABSTRACT: Viral infection of the central nervous system (CNS) is linked to neurodegenerative processes, but the metabolic mechanisms influencing antiviral responses and neuroinflammation remain poorly understood. Nicotinamide adenine dinucleotide (NAD) plays a key role in mitochondrial function and immune signaling, and supplementation with its precursor nicotinamide mononucleotide (NMN) has been proposed as a potential intervention. To explore how NMN modulates cellular responses during CNS viral infection, we performed quantitative LC–MS/MS proteomic profiling in murine Neuro-2a neuroblastoma cells (n=3) and RAW264.7 macrophages (n=4), which are permissive to Theiler’s murine encephalomyelitis virus (TMEV, DA strain, MOI = 1, 24 h). Cells were treated with NMN at 100 µM or 500 µM, with or without TMEV infection. Analysis focused on predefined metabolic and immune-related proteins to assess protein-level changes associated with NAD metabolism and antiviral pathways. Selected differentially expressed proteins were validated independently at the mRNA level by RT–PCR. This dataset includes raw LC–MS/MS data supporting the proteomic findings.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Raw-264.7 Cell, Neuro-2a Cell

SUBMITTER: Cong Thanh Nguyen  

LAB HEAD: Ikuo Tsunoda

PROVIDER: PXD075207 | Pride | 2026-09-29

REPOSITORIES: Pride