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Competitive activity-based protein profiling of LF-268 target engagement in live HCT-116 cells using a click handle-derivatized RAF inhibitor probe

ABSTRACT: Activity-based protein profiling using 268-TCO, a trans-cyclooctene (TCO)-derivatized analogue of the pan-RAF DFG-out inhibitor LF-268, to characterize intracellular target engagement in live HCT-116 (mutant KRAS/WT RAF) cells. Cells were pre-treated with LF-268 (5 µM) or DMSO as competitor, followed by treatment with 268-TCO probe. After lysis, probe-bound protein complexes were captured via click chemistry using tetrazine (Tz)-coupled sepharose beads. Enriched proteins were identified and quantified by label-free LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

SUBMITTER: Daniel Brush

LAB HEAD: Dustin James Maly

PROVIDER: PXD075291 | Pride | 2026-04-20

REPOSITORIES: Pride

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			Action	DRS
	2-112_Co-CP_EphA2_HCT-116_TCO_neg-4hrs_1.raw	Raw
	2-112_Co-CP_EphA2_HCT-116_TCO_neg-4hrs_2.raw	Raw
	2-112_Co-CP_EphA2_HCT-116_TCO_neg-4hrs_3.raw	Raw
	2-112_Co-CP_EphA2_HCT-116_TCO_withcompetitor-4hrs_1.raw	Raw
	2-112_Co-CP_EphA2_HCT-116_TCO_withcompetitor-4hrs_2.raw	Raw

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