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Phosphoproteomic profiling of kinobead-enriched fractions from LF-268-treated HCT-116 cells via SILAC-based quantification

ABSTRACT: SILAC-based phosphoproteomic profiling of kinase-enriched fractions from HCT-116 cells treated with the DFG-out pan-RAF inhibitor LF-268 to characterize kinase signaling responses. Cells were metabolically labeled and treated with 1 µM LF-268 or DMSO for 1 hour, followed by serial kinobead enrichment and Fe³⁺-NTA IMAC phosphopeptide enrichment prior to LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

SUBMITTER: Daniel Brush

LAB HEAD: Dustin James Maly

PROVIDER: PXD075307 | Pride | 2026-04-20

REPOSITORIES: Pride

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			Action	DRS
	111718_1uM_LF-268_1hr_phosphokinomics_w-Annotation.xlsx	Xlsx
	111718_HCT116_L-DMSO_H-1uM-LF-268_1hr_Phospho_tech1.raw	Raw
	111718_HCT116_L-DMSO_H-1uM-LF-268_1hr_Phospho_tech2.raw	Raw
	111718_HCT116_L1-1uM-LF-268_1hr_H1-DMSO_Phospho_tech1.raw	Raw
	111718_HCT116_L1-1uM-LF-268_1hr_H1-DMSO_Phospho_tech2.raw	Raw

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