Dataset Information

Mitochondrial super complexes in the colorless chlorophycean alga Polytomella parva

ABSTRACT: Chlorophycean algae of the genera Chlamydomonas and Polytomella share a common photosynthetic ancestor. However, members of the Polytomella lineage have adopted a heterotrophic lifestyle, having lost the photosynthetic apparatus and relying instead on acetate or ethanol as carbon sources, with energy production centered on oxidative phosphorylation (OXPHOS). In this study, we investigated the composition of the mitochondrial supercomplexes of the colorless alga Polytomella parva. Mitochondrial membranes were solubilized using mild detergents such as glycol-diosgenin and digitonin, followed by separation of OXPHOS complexes supramolecular assemblies via Blue Native electrophoresis and Fast Protein Liquid Chromatography (FPLC). Additionally, complexome profiling of solubilized algal mitochondria resolved by Blue Native Gel Electrophoresis was carried out. The resulting data indicate that the OXPHOS supercomplexes of Polytomella closely resemble those observed in situ in the mitochondria of its green relative Chlamydomonas reinhardtii, as revealed by electron cryo-tomography and subtomogram averaging.

INSTRUMENT(S):

ORGANISM(S): Polytomella Parva

SUBMITTER: Alfredo Cabrera-Orefice

LAB HEAD: Prof. Dr. Diego González-Halphen

PROVIDER: PXD075371 | Pride | 2026-06-01

REPOSITORIES: Pride

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			Action	DRS
	P2024_010_CP_Pparva_Mitos_DDM_01.raw	Raw
	P2024_010_CP_Pparva_Mitos_DDM_02.raw	Raw
	P2024_010_CP_Pparva_Mitos_DDM_03.raw	Raw
	P2024_010_CP_Pparva_Mitos_DDM_04.raw	Raw
	P2024_010_CP_Pparva_Mitos_DDM_05.raw	Raw

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Mitochondrial super complexes in the colorless chlorophycean alga Polytomella parva.

Ostolga-Chavarría Marcos M Rico-Luna Anaiza A Fuentes-Hernández Sergio S Miranda-Astudillo Héctor V HV Cabrera-Orefice Alfredo A González-Halphen Diego D

Journal of bioenergetics and biomembranes 20260507 1

Chlorophycean algae of the genera Chlamydomonas and Polytomella share a common photosynthetic ancestor. However, members of the Polytomella lineage have adopted a heterotrophic lifestyle, having lost the photosynthetic apparatus and relying instead on acetate or ethanol as carbon sources, with energy production centered on oxidative phosphorylation (OXPHOS). In this study, we investigated the composition of the mitochondrial supercomplexes of the colorless alga Polytomella parva. Mitochondrial m ...[more]

PMID: 42209873

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