Dataset Information

Proteomics of Mouse Testicular Organoids on Day 6

ABSTRACT: To investigate the mechanism for the formation of tubule-like structures in testicular organoids, we conducted proteomic analysis on O-Torgs and T-Torgs at day 6. Through differential protein expression and enrichment analysis, it was found that the extracellular matrix (ECM) related pathways in O-Orgs were significantly upregulated. This indicates that the ECM might facilitate the formation of tubule-like structures in O-Orgs, which might be important for spermatogenesis.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Qi Li

LAB HEAD: Xiao-Yang Zhao

PROVIDER: PXD075407 | Pride | 2026-03-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	O-Torgs_Day6_rep1.d.zip	Other
	O-Torgs_Day6_rep2.d.zip	Other
	O-Torgs_Day6_rep3.d.zip	Other
	Spectronaut_O-Torgs_Day6_rep1.m.zip	Other
	Spectronaut_O-Torgs_Day6_rep2.m.zip	Other

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Project description:ITo derive a scaffold from human testis for the purpose of tissue engineering and regenerative medicine, we developed a method to produce a cytocompatible decellularized testicular matrix (DTM) while maintaining the native tissue-specific characteristics and components. The potential benefits of tissue-specific scaffolds consisting of naturally-derived extracellular matrix (ECM) have been demonstrated using a wide variety of animal and human tissue sources. However, so far, testis scaffolds have never been considered for constructive remodelling purposes. We have therefore developed a protocol for the preparation of cell-free extracellular matrix and characterized the material extensively using a combination of proteomics, immunohistochemsitry, and cell population assays. To prepare cell-free matrix, human cadaveric testicular tissue was exposed for 24 h or 48 h to 1% Triton X-100 and/or 1% sodium dodecyl sulfate (SDS). The extent of decellularization was evaluated by histology. Confirmation of cell removal in DTM was done by a DNA quantification technique. The protein composition was analysed by LC-MS/MS. The retention of testicular tissue-specific characteristics was evaluated by immunohistochemistry, Alcian blue staining and scanning electron microscopy. Soluble toxicity and testicular cell attachment was assessed to check the cytocompatibility of DTM scaffolds. Histological analysis showed that DTM could be obtained by mechanical agitation in 1% SDS for 24 h. The resulting DTM was found to be clear of cells while retaining the typical three-dimensional structure. Proteomics analysis revelaed the presence of the major components of the native tissue scaffold, including collagen type I and IV, fibronectin, laminin and glycosaminoglycans. In addition, numerous additional ECM proteins in DTM were detected, indicating its complex nature. Importantly, we demonstrated that DTM scaffolds are not cytotoxic, as evidenced by MTT assay showing a normal fibroblast proliferation activity after indirect exposure, and support testicular cell attachment and infiltration.

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Proteomics of Mouse Testicular Organoids on Day 6

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