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Surface and global proteome profiling of osteosarcoma patient derived xenografts

ABSTRACT: This study analyzed 22 unique OS patient-derived xenografts (PDXs) using proteomics to identify surface proteins for potential immunotherapeutic targeting. Plasma membranes were enriched using density gradient ultracentrifugation and compared to a reference standard of 12 immortalized non-OS cell lines prepared in a similar manner. In parallel, global proteome analysis was carried out on all samples to place the identified surface proteins within the context of deregulated OS pathways. All models were analyzed by Tandem Mass Tags (TMT)-based mass spectrometry to quantify identified proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Gian Luca Negri

LAB HEAD: Gregg Morin

PROVIDER: PXD075484 | Pride | 2026-07-28

REPOSITORIES: Pride

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			Action	DRS
	28122_U54_Sorensen_OsteoSurfaceEnriched_Plex1.raw	Raw
	28122_U54_Sorensen_OsteoSurfaceEnriched_Plex2.raw	Raw
	28122_U54_Sorensen_OsteoSurfaceEnriched_Plex3.raw	Raw
	28122_U54_Sorensen_OsteoSurfaceEnriched_Plex4.raw	Raw
	28122_U54_Sorensen_OsteoSurfaceEnriched_Plex5.raw	Raw

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