Deciphering NAC53, NAC78 interactome in response to Pst infection; NAC53, NAC78, MDP25 subcellular interactomes and identifying ubiquitination sites of NAC53 and NAC78 upon HRD1 E3 ligase mediated ubiquitination.

ABSTRACT: Analysis of NAC53 and NAC78 interactome in normal condition or upon Pseudomonas syringea pv. tomato DC3000 ∆HopQ infection. Constructs promUBQ10::eGFP-NAC53/eGFP-NAC78 are used as baits and promUBQ10::eGFP is used as control. Analysis of ubiquitination sites on NAC53 and NAC78 upon HRD1a/b ubiquitination. Analysis of NAC53, NAC78 and MDP25 subcellular interactome. For NAC53 and NAC78, dual tagged constructs pLjUBQ1::mCerrulean-NAC53/78-mCherry were used to pull-down N-ter moieties (cytosolic/nuclear localization) or C-ter moieties (endoplasmic reticulum localization). pUBQ10::eGFP and p35S::mCherry-HDEL are used as controls. For MDP25, protein variants were used, MDP25FL (plasma membrane localization), MDP25∆N25 (cytosolic localization) or N25MDP25 (plasma membrane localization). pUBQ10::eGFP is used as control.

INSTRUMENT(S):

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Bacterial Infectious Disease

SUBMITTER: Mirita Franz-Wachtel  

LAB HEAD: Suayb Üstün

PROVIDER: PXD075538 | Pride | 2026-03-23

REPOSITORIES: Pride