Dataset Information

Identification of p115 interacting proteins from HEK293T cells

ABSTRACT: The protein p115 is a key player in the delivery of vesicles from the endoplasmic reticulum to the early Golgi. The protein is a homodimer with a folded head domain and a coiled-coil tail that is anchored to Golgi membranes. p115 has been shown to capture vesicles and to bind to SNARE proteins to promote membrane fusion. This project was to look for more interaction partners of p115 by expressing GFP fusions to the full length protein or to either the head or the tail alone. These fusions were expressed in HEK293T cells and co-precipitating proteins identified by mass-spectrometry and compared to those found with precipitation of GFP alone.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Catarina de Matos Ferraz Franco