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Identification of protein complexes in Gonadotropin signaling using discovery proteomics

ABSTRACT: We utilized discovery proteomics to identify putative factors that are recruited to the GnRH-responsive promoter regions of gonadotropin β genes (Egr1 and cFos). We also used this approach to identify proteins that associate with immediate early genes that regulate LHβ and FSHβ.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Gonadtroph, Cell Culture

SUBMITTER: Brandon Le

LAB HEAD: Djurdjica Coss

PROVIDER: PXD075636 | Pride | 2026-06-29

REPOSITORIES: Pride

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			Action	DRS
	CoIP-proteinGroups.renames.3br.txt	Txt
	Ctrl_Egr1_1.raw	Raw
	Ctrl_Egr1_2.raw	Raw
	Ctrl_Egr1_3.raw	Raw
	Ctrl_FSH_1.raw	Raw

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Publications

Discovery proteomics identification of factors contributing to gonadotropin β expression.

Ruggiero-Ruff Rebecca E RE Le Brandon H BH Coss Djurdjica D

Endocrinology 20260625

Luteinizing hormone (LH) and follicle-stimulating hormone (FSH), critical for reproduction, are heterodimers of a common α subunit and unique β subunits, that are limiting components in the mature hormone synthesis. GnRH from the hypothalamus regulates synthesis of LH and FSH β-subunits. Previous studies identified signaling pathways and transcription factors that mediate GnRH induction of β-subunits. However, these studies do not fully explain gonadotrope responsiveness to GnRH or mechanisms of ...[more]

PMID: 42343567

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