Dataset Information

Stress-Specific Carbonylation and Proteasome 20S Activity in Potato under Drought, Elevated Temperature, and Combined Stresses: Linking Oxidative Damage to Proteome Regulation

ABSTRACT: This study investigates stress-specific patterns of protein carbonylation and proteome re-modelling in potato leaves subjected to drought, elevated temperature, and their combina-tion. The research was conducted at the Department of Potato Agronomy of the Plant Breeding and Acclimatization Institute – National Research Institute. The Polish potato cultivar Solanum tuberosum L. ‘Lech’, known for its sensitivity to soil drought, was used in the study.Two weeks after tuber initiation (approximately BBCH 40), plants were subjected to four experimental treatments for a duration of 14 days: Control (C): maintained optimal hydration (70% field water capacity, FWC) and standard temperature conditions (20/16°C day/night). Drought (D): water withheld to reach and maintain 40% FWC at 20/16°C. Elevated Temperature (HT): plants exposed to 38/25°C (day/night) under optimal hydration (70% FWC). Combined Stress (D+HT): plants exposed to 38/25°C (day/night) with water withheld to 40% FWC.

INSTRUMENT(S):

ORGANISM(S): Solanum Tuberosum (potato)

TISSUE(S): Leaf

SUBMITTER: Marta Gietler

LAB HEAD: Marta Gietler

PROVIDER: PXD075691 | Pride | 2026-04-06

REPOSITORIES: Pride

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Stress-Specific Carbonylation and Proteasome 20S Activity in Potato Under Drought, Elevated Temperature, and Combined Stresses: Linking Oxidative Damage to Proteome Regulation.

Boguszewska-Mańkowska Dominika D Fidler-Jarkowska Justyna J Gietler Marta M Nykiel Małgorzata M

Plants (Basel, Switzerland) 20260319 6

Drought and elevated temperature are major abiotic stresses that limit potato growth and productivity; however, their combined effects on biomass and oxidative damage to proteins remain poorly understood. We investigated individual and interactive effects of drought and elevated temperature on growth traits, yield, protein carbonylation, 20S proteasome activity, and the leaf proteome. Results show that while an elevated temperature alone did not significantly impair vegetative biomass or yield, ...[more]

PMID: 41901459

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Project description:The experiment was carried out during two vegetation seasons from 25th of April to 10th of September. Two Polish potato cultivars, the drought-tolerant Gwiazda and drought-sensitive Oberon were used. Selected tubers with transverse diameters of 3 - 4 cm were pre-sprouted for 2 weeks before planting. Plants were grown in a vegetation hall in pots filled with a thin layer of gravel on the bottom and the universal vegetable soil substrate ‘Hollas’ (Agaris Polska Ltd., Poland) produced from peat with the addition of chalk at a pH range of 5.5-6.5. Additionally, in phase 20 of the BBCH-scale of plant development, MIS-3 (Intermag) fertilizer was applied. Water content (WC) in volumetric basis in soil pots was measured according Black (1965). Weather conditions during the years of study were monitored using a Weather Campbell Station (Campbell Scientific Inc.) located in close proximity, and a thermohygrograph placed between pots. Meteorological data of air temperature, the photosynthetically active radiation, and humidity were comparable in the years of study and favorable for potato development. Three weeks after the initiation of the tuberisation phase (56 DAP), plants were divided into 3 groups, each consisting of 6 plants. The first group of plants was subjected to soil drought (remained without irrigation, day/night temperature 22°C/18°C), the second one to high temperature (day/night temperature 38°C/25°C) and the third one was watered according to needs and at optimal temperature (control plants, day/night temperature 22°C/18°C). Stress application lasted 14 days and finished at 70 DAP. During this period, plants were placed in phytotron equipped with six Hortilux Schreder Lamps with Philips light bulbs of 1600 W each. Air humidity was in the range 65-70%. WC under 14 days of soil drought reached 30% (v/v), and remained 80% (v/v) in control and high temperature conditions. During the recovery period, after 14 days of stress treatment, WC reached control levels. Plant root material for proteomic research was collected on the 14th day of stress treatment (70 DAP).

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