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Sucrose-Activated TOR and phyA Signaling Alleviates Shade-Mediated Inhibition of Leaf Development in Arabidopsis

ABSTRACT: To investigate how the sucrose-TOR signaling pathway regulates phyA, we conducted LC-MS/MS analysis to identify phosphorylation sites on phyA. In vivo, the phyA protein was immunoprecipitated from 35S::PHYA-YFP/phyA-211 seedlings treated with shade and 30 mM sucrose for 6 hours. In vitro, we used recombinant phyA protein purified from insect cells as the substrate and endogenous TOR complex immunoprecipitated from the 35S::LST8-1-FLAG transgenic line as the kinase source. Phosphopeptide enrichment and LC–MS/MS analysis of the in vitro reaction were performed.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf, Shoot

SUBMITTER: Jiayu Wang

LAB HEAD: Lin Li

PROVIDER: PXD075951 | Pride | 2026-03-21

REPOSITORIES: Pride

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			Action	DRS
	Exploris05530-SKY-LL-WJY-20250317-phyA-pho.pdResult	Other
	Exploris05530-SKY-LL-WJY-20250317-phyA-pho.raw	Raw
	HT-SKY-LL-WJY-20250106-phyA-vitro-pho_Slot1-68_1_5122.d.zip	Other
	HT-SKY-LL-WJY-20250106-phyA-vitro-pho_Slot1-68_1_5122_6.1.213.pdResult	Other
	HT-SKY-LL-WJY-20250106-phyA-vitro_Slot1-69_1_5123.d.zip	Other

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