Assembly and Reactions of Artificial Metalloenzymes in Streptomyces albus

ABSTRACT: Artificial metalloenzymes (ArMs) expand the suite of synthetically valuable, new-to-nature biocatalytic reactions. Integrating these enzymes into biosynthetic pathways enables reactions not found in nature to occur in living cells with the intermediates or products of metabolic pathways. However, the integration of reactions catalyzed by ArMs into complex metabolic pathways is constrained by the lack of methods to assemble these ArMs in organisms commonly used for metabolic engineering. We report the assembly of an iridium-containing artificial metalloenzyme (Ir-ArM) in Streptomyces albus, a Gram-positive bacterial chassis widely used for the heterologous expression of natural products. In this engineered organism, the Ir-ArM assembles in the cytoplasm and catalyzes abiological carbene transfer to the unactivated, disubstituted double bond of an exogenously added terpene with turnover numbers (TONs) that are two times higher than those for the same reaction catalyzed within E. coli cells harboring Ir-ArM and twenty-times higher than the TONs for the same reaction catalyzed by the purified holoprotein itself.

INSTRUMENT(S):

ORGANISM(S): Streptomyces Albus Subsp. Albus Dsm 41398

SUBMITTER: Christopher Petzold  

LAB HEAD: Christopher Petzold

PROVIDER: PXD076107 | Pride | 2026-10-15

REPOSITORIES: Pride