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Abundance analysis of chromatin-residing proteins during lineage conversion using protein aggregation capture (PAC)

ABSTRACT: We performed abundance analysis of chromatin-residing proteins in BLaER1 cells during the early stages of B-cell-to-macrophage transdifferentiation. We employed protein aggregation capture (PAC) to analyze chromatin-residing protein abundances at 0, 6 and 24 h after induction of transdifferentiation in the absence and presence of the small-molecule SUMOylation inhibitor ML-792 (SUMOi). Mass spectrometry analyses were executed with high resolution mass spectrometry (LC-MS), using the Evosep One liquid chromatography system coupled to Orbitrap Astral mass spectrometer. Raw Orbitrap Astral data-independent acquisition (DIA) data were processed with DIA-NN. Data ouput from DIA-NN were further processed with ProteoGyver.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Suspension Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Emma Valima

LAB HEAD: Jorma J. Palvimo

PROVIDER: PXD076495 | Pride | 2026-05-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	OA_4580_PAC_BLaER1_0hE2_DMSO_1.raw	Raw
	OA_4581_PAC_BLaER1_0hE2_DMSO_2.raw	Raw
	OA_4582_PAC_BLaER1_0hE2_DMSO_3.raw	Raw
	OA_4584_PAC_BLaER1_0hE2_SUMOi_1.raw	Raw
	OA_4585_PAC_BLaER1_0hE2_SUMOi_2.raw	Raw

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