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IWS1 stimulates transcription elongation

ABSTRACT: Transcription elongation requires the coordination of many factors, but the molecular role of these factors are not fully understood. We used cryo-electron microscopy and in vitro RNA extension assays to show that the histone chaperone IWS1 stimulates transcription using a series of short motifs in its C-terminus that contact separate components of the elongation machinery.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Della Syau

LAB HEAD: Lucas Farnung

PROVIDER: PXD076498 | Pride | 2026-06-08

REPOSITORIES: Pride

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Publications

Structure and function of IWS1 in transcription elongation.

Syau Della D Steinruecke Felix F Roth Sophie S Schmid Ernst E Adelman Karen K Walter Johannes C JC Farnung Lucas L

Nucleic acids research 20260501 9

Transcription elongation by RNA polymerase II is a tightly regulated process that requires coordinated interactions between transcription elongation factors. IWS1 (Interacts with SPT6) has been implicated as a core elongation factor, but its molecular role remains unclear. We show that the intrinsically disordered C-terminal region of IWS1 contains short linear motifs (SLiMs) that multivalently engage the elongation machinery. Using cryo-electron microscopy, we map SLiMs in IWS1 that interact wi ...[more]

PMID: 42134803

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