QChIP-MS reveals the local chromatin composition by label-free quantitative proteomics

ABSTRACT: Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combine ChIP with label-free quantitative mass spectrometry (qChIP-MS) to interrogate local chromatin compositions. We demonstrate the versatility of our approach at telomeres, with transcription factors, in tissue and by dCas9-mediated locus-specific enrichment.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Osteosarcoma

SUBMITTER: Dennis Kappei  

LAB HEAD: Dennis Kappei

PROVIDER: PXD076520 | Pride | 2026-04-03

REPOSITORIES: Pride