Proteomic profile of hippocampal growth cones through early postnatal development

ABSTRACT: During early postnatal development, hippocampal circuits are assembled through the coordinated navigation of axonal growth cones toward their synaptic targets. While growth cones are known to rely on local proteomic changes to interpret guidance cues, the developmental dynamics of their proteomic composition remain poorly understood. We performed a systematic proteomic characterization of growth cones isolated from whole Hippocampus or Dentate gyrus tissue at postnatal day (P) 1, 3, and 5 from wildtype animals. Our analysis showed that at P1 growth cones of both tissues have a common proteomic signature. While hippocampal growth cones undergo a rapid transition from exploratory to a more mature state until P5, growth cones of the Dentate Gyrus maintain their exploratory profile in the same time frame suggesting a delayed maturation. Growth cones of the Hippocampus and Dentate Gyrus are characterized by several temporally regulated proteins, reflecting their dynamicity and different developmental trajectories.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Maike Krause  

LAB HEAD: Giulia Quattrocolo

PROVIDER: PXD076616 | Pride | 2026-06-11

REPOSITORIES: Pride