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PSMB4 pulldown ID with PSMD5 overexpression

ABSTRACT: For PSMD5 over expression experiment, PSMB4-tagged cells were transfected in either 6-well plates (Cat. No. 3506; Costar) or 10 cm dishes using 1 µg or 5 µg of HA-PSMD5 or HA-PSMD5ΔCT, respectively. All the transfections were performed with Lipofectamine 3000 (Cat. No. L3000015; Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Cat. No. 31985070; Life Technologies), following the manufacturer’s protocol. Cells were incubated at 37 °C in a humidified atmosphere containing 5 % CO₂ for 48 h before harvesting.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kitaik Lee

LAB HEAD: Francis J.O'Reilly

PROVIDER: PXD076795 | Pride | 2026-07-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	E250423_01_KL_PSMB4_pulldownID_OX_Control_rep1_inj1.raw	Raw
	E250423_02_KL_PSMB4_pulldownID_OX_Control_rep1_inj2.raw	Raw
	E250423_04_KL_PSMB4_pulldownID_OX_Control_rep2_inj1.raw	Raw
	E250423_05_KL_PSMB4_pulldownID_OX_Control_rep2_inj2.raw	Raw
	E250423_07_KL_PSMB4_pulldownID_OX_Control_rep3_inj1.raw	Raw

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