Dataset Information

Native MS and intact-protein MS datasets for NreB and NreB_PAS supporting the study “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”

ABSTRACT: This ProteomeXchange dataset contains native mass spectrometry and intact-protein mass spectrometry data generated in support of the manuscript “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”. The dataset includes intact-protein MS measurements of purified His_NreB and His_NreB_PAS for molecular-mass verification, as well as native MS measurements of NreB_PAS under untreated, GSH-treated, GSH plus NaHS-treated, and pH-dependent conditions. These data were used to characterize Fe-S cluster-associated adduct states and to compare the distributions of species detected under different conditions. Raw data acquired on a Bruker Impact HD mass spectrometer and the corresponding deconvolution outputs generated using Bruker DataAnalysis 4.2 are included together with a deconvolution summary table.

INSTRUMENT(S):

ORGANISM(S): Bacillus Licheniformis Dsm 13 = Atcc 14580

SUBMITTER: chao tang

LAB HEAD: Yongzhen Xia

PROVIDER: PXD076861 | Pride | 2026-04-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	His_NreB_DECONV.d.zip	Other
	His_NreB_PAS_DECONV.d.zip	Other
	His_NreB_PAS_RAW.d.zip	Other
	His_NreB_RAW.d.zip	Other
	NreB_NreBPAS_Deconvolution_Summary_Table.xlsx.xlsx	Xlsx

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Dataset Information

Native MS and intact-protein MS datasets for NreB and NreB_PAS supporting the study “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”

Dataset's files

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