Native MS and intact-protein MS datasets for NreB and NreB_PAS supporting the study “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”

ABSTRACT: This ProteomeXchange dataset contains native mass spectrometry and intact-protein mass spectrometry data generated in support of the manuscript “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”. The dataset includes intact-protein MS measurements of purified His_NreB and His_NreB_PAS for molecular-mass verification, as well as native MS measurements of NreB_PAS under untreated, GSH-treated, GSH plus NaHS-treated, and pH-dependent conditions. These data were used to characterize Fe-S cluster-associated adduct states and to compare the distributions of species detected under different conditions. Raw data acquired on a Bruker Impact HD mass spectrometer and the corresponding deconvolution outputs generated using Bruker DataAnalysis 4.2 are included together with a deconvolution summary table.

INSTRUMENT(S):

ORGANISM(S): Bacillus Licheniformis Dsm 13 = Atcc 14580

SUBMITTER: chao tang  

LAB HEAD: Yongzhen Xia

PROVIDER: PXD076861 | Pride | 2026-04-15

REPOSITORIES: Pride