Dataset Information

Blue-enriched LED light modulates biochemical and proteomic traits without affecting yield in indoor-grown cress microgreens

ABSTRACT: Light spectral composition is a key driver of plant growth and quality in controlled-environment agriculture. This study evaluated the effects of two LED light spectra differing in blue, green, red, and far-red proportions on growth, nutritional traits, and proteomic profiles of indoor-grown cress (Lepidium sativum L.) microgreens. Plants were cultivated under two B:G:R:FR ratios (13:15:61:11 and 24:12:56:8) at identical photosynthetic photon flux density (255 μmol m⁻² s⁻¹). Growth parameters, including plantlet height, fresh and dry weight, dry matter percentage, and yield, did not differ significantly between treatments. Similarly, nitrate content, total chlorophylls, total carbon, total nitrogen, and the C/N ratio remained unchanged. In contrast, the blue-enriched and far-red-reduced spectrum significantly enhanced secondary metabolite accumulation, increasing anthocyanin concentration and phenolic index rising by 77% and 52%, respectively. Microgreen proteomics revealed a general plant reprogramming depending on LED light irradiation, mostly involving components associated with photosynthesis, protein physical control/biosynthesis/homeostasis/modification, multi-process regulation, RNA biosynthesis, vesicle trafficking, redox homeostasis or with unknown function, which showed over-or down-represented trends according to their nature. Notably, these molecular adjustments occurred without compromising growth or productivity. Overall, these results demonstrate that targeted manipulation of LED light quality can enhance the functional and nutritional quality of microgreens through proteomic reprogramming while maintaining yield, highlighting light spectrum modulation as a promising strategy for sustainable indoor and vertical farming systems.

INSTRUMENT(S):

ORGANISM(S): Brassica Napus (rape)

TISSUE(S): Plant

SUBMITTER: Simonetta Caira

LAB HEAD: Simonetta Caira

PROVIDER: PXD077094 | Pride | 2026-05-18

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Brassica_2024.fasta	Fasta
	Bulgari_Shelf_1A_Tray1_1_rep1.raw	Raw
	Bulgari_Shelf_1A_Tray1_1_rep2.raw	Raw
	Bulgari_Shelf_1A_Tray1_1_rep3.raw	Raw
	Bulgari_Shelf_1A_Tray1_2_rep1.raw	Raw

Items per page:

1 - 5 of 46

Publications

Blue-enriched LED light modulates biochemical and proteomic traits without affecting yield in indoor-grown cress microgreens.

Ertani Andrea A Esposito Mariapia M Caira Simonetta S Riahi Jouhaina J Colombani Carla C Scaloni Andrea A Paradiso Roberta R Bulgari Roberta R

Frontiers in plant science 20260427

Light spectral composition is a key driver of plant growth and quality in controlled-environment agriculture. This study evaluated the effects of two LED light spectra differing in blue, green, red, and far-red proportions on growth, nutritional traits, and proteomic profiles of indoor-grown cress (Lepidium sativum L.) microgreens. Plants were cultivated under two B:G:R:FR ratios (13:15:61:11 and 24:12:56:8) at identical photosynthetic photon flux density (255 μmol m-2 s-1 ...[more]

PMID: 42125617

Similar Datasets

Project description:Tomato (Solanum lycopersicum) is a model crop for studying development regulation and ripening in flesh fruits and vegetables. Supplementary light to maintain the optimal light environment can lead to the stable growth of tomatoes in greenhouses and areas without sufficient daily light integral. Technological advances in genome-wide molecular phenotyping have dramatically enhanced our understanding of metabolic shifts in the plant metabolism across tomato fruit development. However, comprehensive metabolic and transcriptional behaviors along the developmental process under supplementary light provided by light-emitting diodes (LED) remain to be fully elucidated. We present integrative omic approaches to identify the impact on the metabolism of a single tomato plant leaf exposed to red LEDs of different intensities during the fruit development stage. Our special light delivery system, the “simplified source-sink model”, involves the exposure of a single leaf below the second truss to red LED light of different intensities. We evaluated fruit-size- and fruit-shape variations elicited by different light intensities. Our findings suggest that more than high-light treatment (500 mmol/m^2/s) with the red LED light is required to accelerate fruit growth for 2 weeks after anthesis. To investigate transcriptomic and metabolomic changes in leaf- and fruit samples we used microarray-, RNA sequencing-, and gas chromatography-mass spectrometry techniques. We found that metabolic shifts in the carbohydrate metabolism and in several key pathways contributed to fruit development, including ripening and cell-wall modification. Our findings suggest that the proposed workflow aids in the identification of key metabolites in the central metabolism that respond to monochromatic red-LED treatment and contribute to increase the fruit size of tomato plants. This study expands our understanding of systems-level responses mediated by low-, appropriate-, and high levels of red light irradiation in the fruit growth of tomato plants.

Project description:We have studied the transcriptional, metabolic and photo-physiological responses to light of different spectral quality in the marine diatom Phaeodactylum tricornutum through time-series studies of cultures exposed to equal doses of photosynthetically usable radiation of blue, green and red light. The experiments showed that short-term differences in gene expression and profiles are mainly light quality-dependent. Transcription of photosynthesis-associated nuclear genes was activated mainly through a light quality-independent mechanism likely to rely on chloroplast-to-nucleus signaling. In contrast, genes encoding proteins important for photoprotection and PSII repair were highly dependent on a blue light receptor-mediated signal. Changes in energy transfer efficiency by light-harvesting pigments were spectrally dependent; furthermore, a declining trend in photosynthetic efficiency was observed in red light. The combined results suggest that diatoms possess a light quality-dependent ability to activate photoprotection and efficient repair of photodamaged PSII. In spite of approximately equal numbers of PSII-absorbed quanta in blue, green and red light, the spectral quality of light is important for diatom responses to ambient light conditions. Continuous, axenic culturing of P. tricornutum was done as described in Nymark et al. (2009). The cultures were incubated at 15M-BM-0C under cool white fluorescent light (Philips TLD 36W/96) providing a scalar irradiance (EPAR) of 100 M-NM-<mol m-2 s-1 under continuous white light (CWL) conditions. Upon the onset of the experiment the cultures were synchronized by 48 h dark-treatment (D48). Thereafter the algae were exposed to blue light (BL), green light (GL) or red light (RL) provided by a waveband specific LED panel (SL3500, Photon Systems Instruments). The algae were exposed to 0.5 h, 6 h or 24 h of: 1) 230 M-NM-<mol m-2 s-1 of RL, 2) 100 M-NM-<mol m-2 s-1 of GL, 3) 50 M-NM-<mol m-2 s-1 of BL or 4) 100 M-NM-<mol m-2 s-1 of WL respectively. Three biological replicas for each of the treatments were harvested. Data for the white light exposure and 48h dark treatment is described in GSE42039, PMID: 23520530.

Project description:The cold acclimation process is regulated by many factors like ambient temperature, day length, light intensity, or hormonal status. Experiments with plants grown under different light-quality conditions indicate that the plant response to cold is also a light-quality-dependent process. Here, the role of light quality in the cold response was studied in one-month-old Arabidopsis thaliana (Col‐0) plants exposed for one week to 4 °C at short‐day conditions under white (100 and 20 μmol m‐2s‐1), blue or red (20 μmol m‐2s‐1) light conditions. An upregulated expression of CBF1, an inhibition of photosynthesis, and an increase in membrane damage showed that blue light enhanced the effect of low temperature. Interestingly, cold-treated plants under blue and red light showed only limited freezing tolerance compared to white light cold-treated plants. Next, the specificity of the light quality signal in cold response was evaluated in Arabidopsis accessions originating from different and contrasting latitudes. In all but one Arabidopsis accessions, blue light increased the effect of cold on photosynthetic parameters and electrolyte leakage. This effect was not found for Ws-0, which lacks functional CRY2 protein, indicating its role in the cold response. Proteomics data confirmed significant differences between red and blue light treated plants at low temperature and showed that the cold response is highly accession specific. In general, blue light increased mainly the cold-stress related proteins and red light induced higher expression of chloroplast-related proteins, which correlated with higher photosynthetic parameters in red light cold-treated plants. Altogether, our data suggest that light modulates two distinct mechanisms during the cold treatment - red light driven cell function maintaining program and blue light activated specific cold response. The importance of mutual complementarity of these mechanisms was demonstrated by significantly higher freezing tolerance of cold-treated plants under white light.